合欢皮总皂苷含量检测方法的建立.docVIP

合欢皮总皂苷含量检测方法的建立 摘要 目的：建立紫外分光光度法测定合欢皮中总皂苷含量。方法：以齐墩果酸为对照品, 进行合欢皮总皂苷的含量测定，并同时进行该方法的可行性分析。结果：合欢皮总皂苷的含量为63.49 %，可行性分析说明，该方法准确度、精密度、稳定性好，加样回收率为107.86 %。结论：本方法操作简便、重现性好,可用于合欢皮总皂苷的含量测定及质量控制。 关键词 合欢皮;总皂苷;齐墩果酸;紫外分光光度法;含量测定 Development of the method of Determination of Total Triterpenoid Saponin in Albizia julibrissin Durazz. Chen Shuang1, Qiu Li-Ying1, Jin Jian2﹡, Feng Lei1 (1. Laboratory of Natural Medicine, School of Medicine and Pharmaceutics, Jiangnan University; 2.The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China) Abstract: Objective Develop a method of determination of total triterpenoid saponin in Albizia julibrissin Durazz by ultraviolet spectrophotometry . Methods The oleanolic acid standard sample was used to make standard curve . Determine the content of total triterpene saponinswas in Albizia julibrissin Durazz , and determine the feasibility analysis of the new method . Results The content of the total triterpene in Albizia julibrissin Durazz is 63.49 %. The feasibility analysis indicated that the new method has good results in acethod is convenient .The method has a good reproducibility , and suitable for the accuracy , precision , stability test . The result of the recovery test is 107.86 % .Conclusion The operation is suiltable for the assayinga and the quality control of the Total Triterpenoid Saponin in Albizia julibrissin Durazz . Key words: silktree albizia bark; triterpenoid saponin ; oleanolic acid; ultraviolet spectrophotometry ; assaying 合欢皮为豆科植物合欢（Albizia julibrissin Durazz.）的干燥树皮，传统上用于治疗心神不安、忧郁失眠、肺痈疮肿、跌扑伤痛等症[1]。目前研究发现合欢皮主要含有三萜皂苷类、　黄酮类、木质素及生物碱等化合物[2、3]，具有显著的镇静催眠、抗肿瘤、抗生育、抗炎症、抗菌、免疫调节等生物活性[4]。近年来有人发现合欢皮总皂苷是一种新型的口服有效的血管生成抑制剂（文章已发表），可有效抑制多种实体瘤的生长，有可能成为临床上防治肿瘤的主要治疗策略之一，因此合欢皮总皂苷的药物制剂研究具有十分重要的现实意义。本文采用紫外分光光度法，建立了快速检测合欢皮提取物中总皂苷含量的检测体系，为合欢皮的新药开发提供了科学的依据。 1 试剂与仪器 （1）材料：齐墩果