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关于有机溶剂提取桉叶精油.pdf

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ISSN 0976 – 3333 Available Online at . International Journal of Pharmaceutical Biological Archives 2010; 1(3):291 - 299 ORIGINAL RESEARCH ARTICLE In Vitro Antibacterial Activity and Phytochemical Analysis of Some Selected Medicinal Plants 1 1 2 1 Pathmanathan M.K , Uthayarasa K , Jeyadevan J.P Jeyaseelan E.C* , 1Department of Botany, Faculty of Science, University of Jaffna, Sri Lanka. 2Department of Chemistry, Faculty of Science, University of Jaffna, Sri Lanka. Received 27 June 2010; Accepted 9 Aug 2010 ABSTRACT Medicinal plants are extensively used in traditional medicine to cure various infectious diseases in human. The present study was undertaken to investigate in vitro antibacterial activity of successively extracted hexane, dichloromethane (DCM), ethyl acetate, ethanol, methanol and aqueous extracts of bulb of Allium sativum , leaf of Eucalyptus citriodora and Ocimum sanctum against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli at different test concentration by agar well diffusion method. The test samples were also subjected to detect the presence of phytochemicals. The results of antibacterial activity was analyzed by using one-way analysis of variance (ANOVA) and followed by Least Significant Difference (LSD) test. The study revealed that the extracts possessed antibacterial activity in a dose dependent manner. Amo