转聚磷激酶基因的大肠杆菌去除水体中的磷.pdfVIP

中国环境科学 2006,26(6) ：742~745 China Environmental Science 转聚磷激酶基因的大肠杆菌去除水体中的磷 王 勤 1,2 2 1 1 1* 1 ,赵庆顺 , 肖 琳 ,蒋丽娟 ,杨柳燕 ,尹大强 (1.南京大学环境学院,污染控制与资源化研究国家 重点实验室,江苏 南京 210093 ；2.南京大学模式动物研究所,医药生物技术国家重点实验室,江苏 南京 210061) 摘要：为探索有效的生物除磷方法,构建了聚磷激酶基因(ppk)的表达载体pET28a(+),并转化大肠杆菌(Escherichia coli)BL21,获得了可过表 达 ppk 基因的工程菌 BL-PPK.RT-PCR 结果表明,ppk 基因在转化的大肠杆菌中得到了高效表达.聚磷试验结果表明,培养 10h 后,转化了 ppk 基因的大肠杆菌细胞内聚磷含量比对照菌株高 20 倍,而培养液中可溶性磷浓度为对照菌株的约 1/9. 关键词：聚磷激酶(PPK) ；聚磷；转基因；大肠杆菌 中图分类号：X172 文献标识码：A 文章编号：1000-6923(2006)06-0742-04 Soluble phosphorus removed by the Escherichia coli transformed with polyphosphate kinase gene. WANG Qin1,2, ZHAO Qing-shun2 1 1 1* 1 , XIAO Lin , JIANG Li-juan , YANG Liu-yan , YIN Da-qiang (1.State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, China ；2.State Key Laboratory of Pharmaceutical Biotechnology, Model Animal Research Center, Nanjing University, Nanjing 210061, China). China Environmental Science, 2006,26(6)：742~745 Abstract ：For exploring an effective technique of phosphorus bioremoval, the express carrier pET28a(+) of polyphosphate kinase (ppk) gene was constructed and transformed as Escherichia coli BL21, and engineering bacteria BL-PPK capable to express ppk gene was obtained. The ppk gene in transformed Escherichia coli obtained highly effective expression. Experiment results of polyphosphate showed that the content of polyphosphate in the Escherichia coli cell transformed to ppk gene was 20 times higher than that in control bacteria after 10 h culture; while in culture liquor, the soluble phosphorus concentration was about 1/9 of the control bacteria. Key words ：polyphosphate kinase(PPK) ；po