D_氨基半乳糖致小鼠_大鼠肝损伤模型的实验性研究_李梅.pdf

86 ACTA ACADEM IAE M ED IC INA E XUZHOU 2007, 27( 2) D - 李 梅, 谷淑玲, 马腾飞 (, 221002) : 目的 D - ( D - G a l) , D - G al , 方法 60 70 SD , D - G al, , , , D - G a l结果 , D - G a l 500 m g / kg , 结论 , D - G a l 500 mg / kg : ; ; D - ; ; : R 965. 1; R575 : A : 1000 - 2065( 2007) 02- 0086- 0 3 Themaking of amodel of hepatic injury induced with D- galactosamine in rats L I M e, GU Shu - l ng, MA T eng- fe ( D epa rtm en t of Pharm aco logy, Xuzhou M ed cal Co llege, Xuzhou, J ang su 22 1002, Ch na) Abstract: Objective T o optm ze the do sag e o f D - ga lactosam ne ( D - G a l) to m ake a m ou se /ratm odel of hepa t c n jury as a too l for the developm ent of hepat c drug s. M ethods The m ode lw as nduced w th d fferent doses of D - G al p n 70 rats and 60 m ce. T he levels o f ALT and A ST w e re determ ned by u s ng autom at c b ochem cal ana lyzer a t 12, 24, 48 and 72 h. L ver spec m ens w ere pro cessed for patho log ca l exam na t on. R esults C om prehens ve ev aluat on o f the results show ed that the u se o f D - G al 500 mg / kg p n rats could nduce stable m odels w th low m orta l ty. Conclu sion T he hepat c njury m ode l nduced w th D - G a l 500 m g / kg p n rats, be tter than that nduced n m ce, s recom m ended fo r hepa t c pharm aceut cal stud es. Key w ords: D - galactosam ne; hepat c njury; experm enta lmode;l m ou se; ra t 1. 1. 1 D - Ga l ( S gm a , ) 10% , 0. 01 m o l/L , N aOH pH 7. 2~ 7. 4 , 1. 1. 2 SD 70 , 180 ~ 220 CC l g;