D_氨基葡萄糖对胶质瘤细胞生长特性影响研究.pdf

2008 4 A pr. 200 8 34 2 JOURNAL OF MODERN CL IN ICA L M ED ICIN E V o l. 34 N o. 2 论著与研究 [ ] 1673- 1557 2008) 02- 0083- 03 [ ] R73- 34 [ ] A D - 1 2 1 2 张 巍, 唐善虎 , 秦文玲 , 孟 丽 1. 成 理工大学, 四川 成 610059; 2. 西南民族大学, 四川 成 610041) [] : 研究 D - 氨基葡萄糖对胶质瘤细胞的生长抑制作用, 并探讨其作用机理: 采用 MTT 法筛选 D - 氨基葡萄糖最佳浓度, 应用流式细胞仪检测 D - 氨基葡萄糖对细胞周期的影响: D - 氨基葡萄糖能明显抑制胶质瘤细胞生长, 并且呈现 一定的剂量依赖性35 m g L- 1 D - 氨基葡萄 糖表现出抑制率高峰, 为 214% 经 D - 氨基葡萄糖作用后细胞在 G2- M 期的比例增加, 说明细胞停 滞在 G2- M 期, 但是未出现凋亡峰: D - 氨基葡萄糖能抑制人胶质瘤细胞的生长, 其作用机理是 使细胞生长停滞在 G2- M 期 [] D - 氨基葡萄糖; 胶质瘤; 抗癌作用; 体外培养 InvestigationofEffects ofD- Glucosamine onGrowthofNeurogliocytomaCells 1 2 1 2 ZHANG W e i, TANG Shan- hu , Q IN W en- ling , MENG L i 1. Chengdu Un iversity of T echno logy, S ichuan Chengdu 610059, Ch ina; 2. Southw estern Un iversity for N ationa lities, S ichuan Chengdu 610041, Ch ina) [Abstract]Objective:To study the effects of D - g lucosam ine on the grow th inhibition and induction of apoptosis in the neurog liocytom a ce lls in v itro. ethods: Ce ll pro liferation w as exam ined by MTT essay to optmi ize D - g lucosam ine concen trat ions. Ce ll cycles w ere detected based on ce ll DNA conten ts by flow cy tom eter FCA ) . Results:D - g lucosam ine had sign ificant inhibition on the grow th of neurog liocy tom a ce lls - 1 in v itro. T he concen trat ion o f D - g lucosam ine w as 35 mg L , and had m ax mi al inh ib it ion effects, the inh ib it ion rat io w as 214% , respect ive l