PAGE检测过氧化物酶同工酶的分析方法.pdf

安徽农业科学袁允燥怎则灶葬造 燥枣 粤灶澡怎蚤粤早则蚤援杂糟蚤援圆园园8袁36渊14冤院5759-5760 袁5766 责任编辑 姜丽 责任校对 马君叶 1 种利用SDS PAGE 检测过氧化物酶同工酶的分析方法 廖海，杜祯，周嘉裕 ，宋良科，靳晓娥，黄丹娥 （西南交通大学药学院，四川峨眉 614202 ） 摘要 目的建立 种能够检测过氧化物酶同工酶的电泳方法。方法利用 分离蛋白质样品分辨率较高的优点，借助 [ ] 1 [ ] SDS PAGE SDS 分离蛋白质样品，电泳完毕后，胶板用 处理，使过氧化物酶同工酶复 ，以醋酸联苯胺染色。结果利用新建立的方 PAGE Triton X 100 [ ] 法发现，种菖蒲属植物含有 条过氧化物酶同工酶带，而利用传统的 分析方法则只显示 条过氧化物酶同工酶带。与 5 6 PAGE 5 PAGE 同工酶检测方法比较，新建立的方法分离效果更好，酶带边界更清晰，是 种分辨率更高的检测方法。结论利用新建立的方法得到 1 [ ] 的过氧化物同工酶电泳图谱可以为菖蒲的科学分类和鉴定提供新的生化指标。 关键词 SDS PAGE ；过氧化物酶；同工酶；菖蒲 中图分类号 文献标识码 文章编号 （ ） S188 A 0517-6611 2008 14-05759-02 A Detecting Method for Analysis of Peroxidase Isoenzymes Using SDS PAGE LIAO Hai et al (College of Pharmacy, Southwest Jiaotong University, Emei, Sichuan 614202) Abstract [Obj ective] The research aimed to establish a detecting method for Peroxidase isoenzymes. [Method] Peroxidase isoenzymes were isolated by SDS PAGE, SDS was removed by washing the gel in Triton X 100, and the peroxidase was stained with acetic acid and benzidine. [Result] There were 5 isoenzyme bands by using PAGE isoenzyme method in5 species of A corus L. But there were 6 bands observed by using the new method. Compared with the common PAGE isoenzyme method, the better separating effect and clearer borderline of bands were observed in the new method. [Conclusion] The electrophoresis pattern of SDS PAGE isoenzyme method for detecting peroxidase could be regarded as peroxidase