两种组织脱钙法对免疫组织化学染色影响比较.pdf

( ) 290 JOURNAL OF PEK ING UN IVERSITY(H EALTH SC IENCES) V ol. 43 No. 2 Apr. 2011 技术方法 两种组织脱钙法对免疫组织化学染色影响比较 杜 娟, 柳剑英 , 苏 静 (, 100 191) [ ] 目的: 1% () 10% () 方法: 3, 9, 3, 10% () 2442 48 h, 1% () 12 3 h, , , ERPR K -i 67, TT F-1, 34E 12 P504s, AE 1/AE3P504s K i-67, CD3CD20 K i-67, CD3CD20CD68CD235 MPO K-i 67AE1/AE3TTF-1结果: , ; , TTF-1 K i-67, ERCD3 CD20结论: , , 10% ( ), 1% () [] ; ; ; [] R36-33 [] A [ ] 167 1-167X ( 2011) 02-0290-05 doi: 10. 3969 / j. issn. 1671-167X. 2011. 02. 025 Comparison of two different tissue decalcificationmethods for immunohistochem istry DU Ju n, LIU Ji n-y ing , SU Jing ( Dep rtm en t of P thology, Peking Un iversity School of B sicM ed ic l Sciences, Beijing 10019 1, Ch in ) ABSTRACT Objective: To investig te the effects of 1% hydroch loric cid nd 10% n itric cid on mi mu- nohistochem ic l st in ing for sever l ntigens. Methods: F ifteen c ses of foc lly c lcified soft tumors in- cluding three c ses of thyroid p pill ry c rcinom , three of bre st inv sive duct l c rcinom , three of prost te c ncer, three of colon denoc rcincom nd three of norm l lymph nodes, w ere selected in th is study, nd so w ere n ine c ses of bone nd bonem rrow tissue from b iopsy nd u topsy. T issue s mples w ere divided in to three groups. One group w s e ch so ked in to 10% nitric cid for 24, 42 nd 48 h, nother group w s e ch incub ted in 1% hydroch loric cid for 1, 2 nd 3 h. The other group w hich w s not tre ted w ith cid w s reg rded s contro.l Then mi munoh istochem ic l st in ingw s conducted in these s mples for n tigens s follow s: ER, PR nd K i-67 in bre st inv sive duct l c rcinom ; TTF-1 in thyroid p pill ry c rcinom ; 34E12 nd P504s in prost te c nce