乳酸菌质粒提取方法的建立.pdf

2008 3 21 3 Chin J Biologicals March 2008, Vol. 21 No . 3 237 Q782 A 2008 1, 2, 3 2 1, 2 2, 3 2, 3 1, 2 马海利 金宁一 李昌 杜桢 阚秋霞 陈晓月 , , 10 mg/ ml, , , ; ; ; Development of A Method for Extraction of lasmid DNA from Lactic Acid Bacteria MA Haili , J N Ningyi, L Chang, et al ( College fo A nimal Science and Veterinary Medicine, Jilin University, Changchun 130062, China) Abstract Objective To develop a simple, rapid and safe method for extraction of plasmid DNA from lactic acid bacteria. Methods Developa method for extraction of plasmid DNA from lactic acid bacteria by combining the method for extraction of plasmid DNA from E. coli and the method reported. Compare the electrophoretic profiles and restriction analysis results of plasmid DNAs extracted by various methods and optimize the lysozyme concentration to evaluate the feasibility of developed method. Results T he electrophoretic profile and restriction analysis result of plasmid DNA extracted by the developed method showed no significant difference with those by the method reported. The optimal lysozyme concentration was 10 mg/ ml. n addition, by the developed method, the usage toxic ethidium bromide was avoid, and both the dosage of lysozyme and volume of solution decreased. Conclusion The developed method may be used for the rapid extraction of plasmid DNA from lac tic acid bacteria,which laid a foundation of molecular biological study on lactic acid bacteria. Key words Lactic acid bacteria; Plasmid; Extraction; Lysozyme ( Lactic acid bacteria, LAB) , , , , [ 1] [ 7] Anderso