交替剪接对干细胞多能性维持与谱系分化的调控.pdf

中国细胞生物学学报 Chinese Journal of Cell Biology 2013, 35(2): 240–246 http ://www.cj 交替剪接对干细胞多能性维持与谱系分化的调控 刘 欢 李 杰 周 跃* (第三军医大学附属新桥医院骨科, 重庆400037) 摘要 交替剪接是转录后修饰的一个重要过程, 它很好的解释了基因数量有限性和蛋白质 多样性之间巨大差异的问题。交替剪接能够调控细胞的多种生物学行为, 比如增殖、分化和发育 等, 而且与许多疾病的发生相关, 包括癌症。干细胞多能性维持和分化的研究大多集中在转录因子、 染色质重塑和非编码RNA上, 交替剪接概念的引入为干细胞研究提供了一个新的视角。该文综述 了干细胞交替剪接调控的必威体育精装版研究, 首先简述了不同类型的干细胞(全能干细胞、多能干细胞和专 能干细胞) 中存在的交替剪接事件; 其次, 从四个方面阐述了交替剪接对干细胞多能性的调控; 最 后, 系统地总结了干细胞向神经组织、肌肉组织、造血系统、脂肪组织和骨组织分化过程中发生 的交替剪接事件。这些研究充分说明了未来干细胞领域的研究中, 交替剪接是不可或缺的一部分。 关键词 交替剪接; 干细胞; 全能性; 谱系分化 Regulation of Stem Cell Pluripotency Maintenance and Cell Lineage Differentiation by Alternative Splicing Liu Huan, Li Jie, Zhou Yue* (Department of Orthopedics, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China) Abstract Alternative splicing is an important process of post-transcriptional modification. It provides a good explanation for the significant difference between the limited number of genes and the protein diversity. Several biological behaviors are regulated by alternative splicing, such as proliferation, differentiation and development. As well as, the occurrence of many diseases are related with alternative splicing, including cancer. The studies of stem cell pluripotency maintenance and cell lineage differentiation mostly focus on transcription factors, chromatin remodeling, and non-coding RNA. Alternative splicing provides a new sight for stem cell study. The text summarized newest researches on regulation of stem cell by alternative splicing. First alternative splicing events were existed in different kinds of stem cells, including totipotent stem cells, pluripotent stem cells and progenitor cells. Next, th