低氧预处理骨髓间充质干细胞耐受缺血缺氧的能力.doc

Hypoxic preconditioning effects on the ability of bone marrow mesenchymal stem cells tolerant to ischemia and hypoxia* Xu Qiao-yan1, Xia Lin2, Wang Ji-chang3 1 Department of Respiratory Medicine, the People’s Hospital of Laizhou, Yantai 261400, Shandong Province, China 2 Department of Plastic Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China 3 Department of Plastic Surgery, the Second Hospital of Shandong University, Jinan 250033, Shandong Province, China Abstract BACKGROUND: According to the previous studies, it is known that bone marrow mesenchymal stem cells live in the physiological environment of lower oxygen concentration compared with the common culture concentration (20%-21%). Hypoxia can promote bone marrow mesenchymal stem cells proliferation and maintain the characteristics of differentiation potential. OBJECTIVE: To understand the biological characteristics of bone marrow mesenchymal stem cells after hypoxic preconditioning, and search for in vitro basis for bone marrow mesenchymal stem cells therapy in vivo with hypoxic preconditioning strategy. METHODS: After passage and inoculation, bone marrow mesenchymal stem cells were cultured in the hypoxic incubator (1% O2, 5% CO2). Bone marrow mesenchymal stem cells cultured in the normoxic incubator (20% O2, 5% CO2) served as controls. RESULTS AND CONCLUSION: Compared with bone marrow mesenchymal stem cells cultured under normoxia (20%O2), bone marrow mesenchymal stem cells cultured under hypoxia (1% O2) began to display exponential growth phase after a longer incubation period. Hypoxia (1% O2) was not the lethal condition for bone marrow mesenchymal stem cells. Cell survival rates of both groups reduced over time, while those of bone marrow mesenchymal stem cells cultured under normoxia reduced faster (P 0.05). Surface markers expression of bone marrow mesenchymal stem cells, including CD29(+), CD90(+), CD31(-), CD45(-), after hypoxic preconditioning did not change gr