利用脂肪酸生物标记法（PLFAs）分析水稻根际土壤微生物多.doc

利用脂肪酸生物标记法分析水稻根际土壤微生物多样性( 摘要：分析脂肪酸生物标记Ⅱ优优制脂肪酸生物标记采用多样性指数Shannon-Wiener）表示根际微生物群落结构多样性并共检测到脂肪酸生物标记，根际土壤中细菌真菌放线菌16:0（细菌）、18:1ω9с（真菌）、10Me l7:0（放线菌）、10Me 16:0（硫酸盐还原细菌16:1ω5с（甲烷氧化菌优制Ⅱ优进行聚类Ⅱ优优制脂肪酸生物标记水稻根际土壤微生物群落结构丰富 关键词：水稻，根际微生物，磷脂脂肪酸Microbial community diversity analysis in the rhizosphere of rice using Phospholipid Fatty Acid biomarkers detection LIU Bo1，HU Gui－ping1，ZHENG Xue－fang1，ZHANG Jian－fu2，XIE Hua－an3* (1 Agricultural Biological Resource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003; 2 Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350018;3 Fuzhou Branch, National Rice Improvement Center of China, Fuzhou 350018) Abstract:【Objective】The goal of the research was to analyze the genetic relationship between rhizosphere microbial community structure and variety of rice by using Phospholipid Fatty Acid biomarkers detection. 【Method】The rhizosphere microbial community of rice were sampled to analyzed by Phospholipid Fatty Acid biomarkers detection, the diversity index (Shannon-Wiener) was introduced into analyzing the microbial community diversity based on PLFAs. The quantities of PLFAs were clustered by SPSS to analyze the characteristics of microbial community in the rice rhizosphere. 【Result】The results showed that 38 PLFAs biomarkers were detected out, in which there were 23 PLFAs completely distributed and 15 PLFAs incompletely distributed in the rice rhizosphere. The quantities of PLFAs related to bacteria were higher than either that of fungi or that of actinomycetes. The results also revealed that the roots’ microbial community structure represented by PLFAs was closely related to the variety biology, in which the relationship between the PLFA and the effective ear was positive, so did the production, whereas the relationship between the PLFA and the plant height was negatively correlated. Simultaneously, the cluster analysis displayed that the PLFAs were concerned with the rice heredity characteristic. 【