包涵体蛋白复性及其影响因素.pdf

24 5 河 北 工 业 科 技 Vol. 24, No . 5 2007 9 H ebei Journal of Industrial Science and T echnolog Sept. 2007 : 2007) , ( 河北化工医药职业技术学院制药工程系, 河北石家庄 050026) : 针对包涵体需经过细胞破碎 性溶解复性处理后才能形成具有一定空间构象的生物活 性蛋白, 且复性处理是一个非常复杂的生化反应过程, 只有选择合理的方法, 控制适宜的条件, 才能 提高蛋白的复性效率, 综述了包涵体蛋白复性的方法, 分析了影响复性的有关因素 : 包涵体; 溶解; 复性; 影响因素 : Q512 : A Wa s and influence factors for renaturation of protein inclusion bodies YU W enguo , TA O Xiue ( Department of Pharmaceutical Engineering , Hebei Chemical and Pharmaceutical Vocational T echnolog College, Shijiazhuang Hebei 050026, China) Abstract: T o form active protein w ith an active conformation, processes such as crushing , dissolving and renaturalizing are needed. T he renat uration is a ver complicated biochemist r process. Onl b selecting the proper w a and controlling suitable conditions can w e raise t he efficienc of protein renaturation. This paper summarizes t he strategies for renaturat ion of inclusion bodies, and anal ses the fact ors that influnce renaturation of protein inclusion bodies. Key words: inclusion bod ; dissolve; renaturat ion; influnce factors 1 , 1) 50% ( ) , ( RNA ompC [ 1] ) , ompF ompA , DNA, , 0. 5~ 1 m, , , ( 1. 3 mg/ mL) , , , 3 [ 2] 2) , ,