F外周血单个核细胞的研究中国实用儿科.docVIP

EB病毒感染外周血单个核细胞的研究 李金颖1 付丽娟2 邸顺祥1 宋晓燕1 朱庆玲1 基金项目：哈尔滨市科学技术局青年科学研究基金（2005AFQQJ044） 作者单位：1.哈尔滨市儿童医院感染科，黑龙江哈尔滨 150001；2.黑龙江省医院感染内科，黑龙江哈尔滨 150036 E-mail: lijinying1972@ [摘要] 目的：应用荧光定量PCR方法检测EB病毒（EBV）感染患儿外周血单个核细胞（PBMC）中的EBV DNA，探讨EBV感染单个核细胞的临床意义。 方法：应用酶链免疫吸附方法（ELISA）和荧光定量PCR方法同步平行检测38名EBV感染患儿发热7天内、起病一个月、三个月、六个月及九个月血浆中EBV VCR-IgM、外周血全血、血浆、单个核细胞（PBMC）中EBV DNA，比较四种检出方式的检出率在EBV感染患儿病程中的变化。 结果：在EBV感染初期，全血和单个核细胞中EBV DNA阳性率最高，与EBV VCR-IgM及血浆EBV DNA相比，具有统计学意义（P0.05）；起病后的一个月、三个月、六个月及九个月，四种检出方式的阳性率均逐渐减低，但单个核细胞中EBV DNA敏感性始终高于其他三种方式，具有统计学意义（P0.05）。 结论：EBV感染初期，用全血和单个核细胞检测EBV DNA，是早期、快速、敏感的检测方法；EBV可长期存在于单个核细胞中，EBV感染患儿表现出多系统损伤可能与PBMC中EBV感染相关；荧光定量PCR方法检测外周血单个核细胞EBV DNA对EBV感染的诊断有极高价值，可作为判断疗效及监测病情的一种有效手段。 [关键词] EB病毒DNA；单个核细胞（PBMC）；全血；血浆；荧光定量PCR Study on Epstein-Barr virus infected in peripheral blood mononuclear cells. Lj Jin-ying1，Fu Li-juan2，Di Shun-xiang1，et al. 1、Department of Infectious Diseases , Children′s hospital of Harbin, 2、Department of Infectious Diseases HeiLongJiang province hospital 150036. Abstract objective：To detect EBV DNA in peripheral blood mononuclear cells (PBMC) of EBV infected children by fluorescence quantitative PCR，and investigate the clinical significance of EBV infected in mononuclear cells. Method: using ELIAS and fluorescence quantitative PCR to synchronously detect EBV VCR-IgM in plasma, EBV DNA in peripheral whole blood, plasma and PBMC of 38 EBV infected children within seven days with fever, one、three、six and nine months from onsend compare the detect rate change of the four detection methods in the course of disease of EBV infection. Result: In the early days of EBV infection, the highest positive rates are EBV DNA in whole blood and PBMC and there is statistical significance compared with EBV VCR-IgM and EBV DNA in plasma（P0.05）. In one, three, six and nine months from onset , the positive rates decrease gradually in the four detection methods, but the sensitivity of EBV DNA in PBMC is higher than the other methods, and with statistical signific