亚健康小鼠实验模型.docVIP

疲劳型亚健康小鼠模型的研制 ? 刘艳艳1 陈淑娟2 黄建华3 谭为1 欧阳明子1赵晓山1 罗仁1# 1南方医科大学附属南方医院中医科（510515）；2广州军区总医院肾内科（510010）；3广州军区总医院呼吸内科（510010） 摘要 目的 研制疲劳型亚健康小鼠模型。方法 实验一：每天强迫小鼠水中站立8h，分别观察连续造模5d、9d、12d、16d时小鼠行为学表现，以力竭游泳时间反映其疲劳程度，结合血浆生化、血常规及病理组织学检查等，确定造成疲劳型亚健康的最佳时间。实验二：对上述创建的疲劳型亚健康小鼠模型的评价，观察模型小鼠的疲劳持续时间。结果 实验一：模型各组游泳时间与正常组比较显著下降， P=0.000。血生化示，仅造模16d组与正常组小鼠在BUN、ALT、AST、TG水平上发生明显改变（Р0.05）。其余血生化、血常规及病理切片未见明显异常。实验二：模型小鼠力竭游泳时间明显下降；去除造模因素，休息恢复7d后其力竭游泳时间虽有增长，但与正常组仍有差异（P0.05）；至恢复14d后，小鼠力竭游泳时间与正常组比较无显著性差异（P0.05）。结论 采用强迫小鼠水站立8h，连续9d，可成功制备疲劳型亚健康小鼠模型。模型评价表明该模型的稳定性、重复性较好。这为疲劳型亚健康病理机制的研究及相关药物的筛选提供了技术平台。 关键词: 疲劳型亚健康 小鼠 动物模型 A study of fatigue-type sub-health model on mice Liu Yan-yan1, Chen Shu-juan 2, Huang Jian-hua3, Tan Wei1, Ou Yang-ming-zi, Zhao Xiao-shan1, Luo Ren1# 1Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515; 2Department of nephrology, General Hospital of Guangzhou Military Command, Guangzhou 510010; 3Department of Respiratory Medicine, General Hospital of Guangzhou Military Command, Guangzhou 510010 Abstract：Objective To establish and evaluate the fatigue-type sub-health model of mice. Methods Experiment 1: Mice in model groups were forced to stand in water 8 hours per day each for 5days, 9days, 12days and 16days. In the course of experiments, we analyzed the performances of mice behaviors, degree of fatigue, pathological signs in tissue, blood biochemistry results and other indicators, to determine the optimal time for this fatigue-type sub-health model. Experiment 2: On the basement of these above working, mice model of fatigue-type sub-health status was prepared. Then we observed their recovery in 7th, 14th day after their model, using the test of 7% weight-loaded forced swim. Results Experiment 1: In comparison with normal control group, the exhaustive swimming time was notably down among model of 5d, 9d, 12d, 16d groups (P=0.000). In terms of blood biochemistry results, the levels of BUN, ALT, AST, TG were different among model