田基黄对荷瘤小鼠抗肿瘤及增效减毒作用的实验研究.docVIP

田基黄提取物与5-FU联合应用治疗肝癌的实验性研究 1谢佐福， 1蔡娜， 2沙枚，1施文荣，3林久茂，1林雅惠 (1福建中医药大学中西医结合学院，2福建中医药大学药学院， 3福建中医药大学中西医结合研究院) 【摘要】目的 研究田基黄提取物对H22荷瘤小鼠的抗肿瘤作用及其对化疗药物氟脲嘧啶（5-FU）的增效减毒作用。材料与方法采用荷H22肝癌小鼠为模型，在体观察田基黄醇提液和水提液单独应用以及与5-FU联合应用对荷瘤小鼠抑瘤率、脾指数和胸腺指数等的影响。结果 田基黄醇体液和水提液对H22移植瘤具有一定的抑制作用，抑瘤率分别为11.49%和21.41%，两者与5-FU联合应用能提高后者对H22移植瘤的抑瘤率，但均无显著意义（P0.05）田基黄醇提取液和水体液也能显著升高H22荷肝癌小鼠的脾指数和胸腺指数。结论 田基黄提取物对肝癌具有一定的抑制作用，对5-FU具有一定的减毒增效作用。 关键词：田基黄 肝癌 5-FU 减毒增效 Experimental study on the anti- Hepatocellular carcinoma effect of Hypericum japonicum Thumb extract and combined with 5-fluorouracil (1Xiezuofu,2 Caina,1 Shamei,etal. 1 Department of Traditional Chinese Medicine ,2 Pharmacy Department ,Fujian University of Traditional Chinese Medicine ) 【Abstract】Objective: To investigate the antitumor effects of Hypericum japonicum Thumb extract on H22 hepatoma-bearing mice and its synergism and attenuation effects on 5-FU. Methods: Transplanted H22 tumor?bearing mice were used as a model for observing the inhibition rate of tumor、thymus index and spleen index of Hypericum japonicum Thumb extracts alone groups and 5-FU combination groups.Results: Hypericum japonicum Thumb ethanol extracts and distilled water extracts can show a satisfactory function of inhibiting transplanted H22 tumor growth，the inhibition rate was 11.49% and 21.41%, the extracts combined with 5-FU can enhanced the ability of 5-FU to inhibit the growth of sarcoma,but showed no difference (P0.05). The thymus index and spleen index were significantly elevated in Hypericum japonicum Thumb extract groups. Conclusion: The results indicate that Hypericum japonicum 本文由福建省自然科学基金课题资助（2009J0101） 作者：谢佐福（1957-），男，生理学教授，病理学主任医师，主要研究方向为肿瘤的中西医结合基础研究。 Thumb extract has antitumor effects in vivo and attenuation effects on 5-FU. 【Key words】Hypericum japonicum Thumb；Hepatocelluar carcinoma；5-fluorouracil；Synergism and attenuation 化学治疗是目前恶性肿瘤综合治疗中较为有效而又普遍使用的方法之一, 但单纯化疗常引起导致严重的毒副作用，如抑制免疫、造血功能、肝肾功能等 [1]。中药在对肿瘤化疗减毒增效上具有独特的优势，是中药治疗肿瘤的一个重要方面