紫杉醇对Lewis肺癌抑制作用及机理的研究.docVIP

紫杉醇对Lewis肺癌抑制作用及机理的研究 文章来源： 　　　　 2006-7-29 10:48:14 ? 紫杉醇对Lewis肺癌抑制作用及机理的研究 中国肺癌杂志 2000年第3期第3卷 实验研究 作者：林宜先　江曼　刘铭球　刁路明　夏冬　杨桂芳 单位：林宜先　杨桂芳（430071　武汉，湖北医科大学附属第二医院病理科）；江曼　刘铭球　刁路明　夏冬（湖北医科大学病理学教研室） 关键词：紫杉醇；Lewis肺癌；抑制作用 　　【摘要】　目的　探讨紫杉醇对Lewis肺癌生长和转移的抑制作用。方法　将Lewis肺癌细胞接种于C57BL／6小鼠皮下，腹腔注射紫杉醇10?mg／kg，连续10?d。处理动物后测量治疗组及对照组皮下瘤重、肺转移灶计数，应用流式细胞术测定肿瘤细胞凋亡率，并进行光镜和电镜观察。结果　对照组和治疗组皮下瘤重分别为(2.833±0.074)g和(0.588±0.040)g(P＜0.01)，肺转移灶计数分别为（5.500±0.926）个和（1.167±0.753）个(P＜0.01)，肿瘤细胞凋亡率分别为9.002％和25.772％(P＜0.01)。电镜观察紫杉醇组可见典型的凋亡细胞和凋亡小体。结论　紫杉醇可明显抑制小鼠Lewis肺癌的生长和转移，并可诱导肿瘤细胞凋亡。 A study of paclitaxel on inhibiting effects and mechanisms for Lewis lung carcinoma LIN Yixian*, JIANG Man, LIU Mingqiu, DIAO Luming, XIA Dong, YANG Guifang. *Department of Pathology, The Affiliated Second Hospital, Hubei Medical University, Wuhan, Hubei 430071, P.R.China 　　【Abstract】　Objective　To research the inhibitory effect and mechanisms of paclitaxel on Lewis lung carcinoma(LLC) of C57BL/6 mice. Methods　LLC cells were inoculated subcutaneously into C57BL/6 mice. Paclitaxel (10?mg/kg) was injected intraperitoneally in the treatment group for ten days. Then the growth and metastasis of LLC were observed and the apoptotic characteristics of LLC cells were detected by electron microscopy and flowcytes assay. Results　The average weights of the tumors in treatment group and control group were (2.833±0.074) g and (0.588±0.040) g (P＜0.01), the average numbers of lung metastasis were 5.500±0.926 and 1.167±0.753 (P＜0.01), and the ratio of apoptosis of LLC cells were 9.002％ and 25.772％(P＜0.01), respectively. Some typical apoptotic cells and apoptotic bodies were observed through electron microscope. Conclusion　Paclitaxel can effectively inhibit the growth, development and metastasis of the implanted LLC in C57BL/6 mice and it can induce apoptosis of the tumor cells apparently. 　　【Key words】　Paclitaxel　　Lewis lung carcinoma　　Inhibiting effect 　　This work was supported in part by a grant from Hubei Natural Science Fund (to Liu M