利用RFLP分析DO对附积床系统中AOB群落结构的影响.pdfVIP

中国环境科学 2014,34(9) ：2387~2393 China Environmental Science 利用 RFLP 分析 DO 对附积床系统中 AOB 群落结构的影响 * 张 岩 ,朱 敏,刘焕光,孙凤侠,甘志明,陈 敬,史 杨,谢杭冀 (北京工业大学,北京市水质科学与水环境 恢复工程重点实验室,北京 100124) 摘要：为了解析 DO 浓度对附积床反应器脱氮系统中 COD、NH4+-N 、TN 去除效率的影响, 以及对氨氧化菌群(AOB)结构及多样性的影响, 分析了 DO 分别为 1.0~2.0,2.0~3.0,3.0~4.0mg/L 时 COD、NH4+-N 、TN 去除效率,并采用针对 AOB 功能基因氨单加氧酶(amoA)的限制性 内切酶片段长度多态性技术(RFLP)分析了三组 DO 浓度下反应器中 AOB 的群落结构及多样性.结果表明,不同 DO 条件下,系统均取得较高 的 COD 和 NH4+-N 的去除效果, NH4+-N 的去除效率随着 DO 的增加而提高.不同 DO 浓度下反应器生物膜上 AOB 菌群多样性丰富,且与 DO 对 AOB 菌群的多样性影响较小相比,DO 对 AOB 的菌群结构及种类的影响较大. 关键词：氨氧化细菌(AOB) ；amoA 基因；RFLP ；生物膜 中图分类号：X172 文献标识码：A 文章编号：1000-6923(2014)09-2387-07 Analysis of ammonia-oxidizing bacteria in catching bed reactor at different DO concentrations by RFLP. ZHANG * Yan , ZHU Min, LIU Huan-guang, SUN Feng-xia, GAN Zhi-ming, CHEN-Jing, SHI-Yang, XIE Hang-ji (Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China). China Environmental Science, 2014,34(9)：2387~2393 Abstract ：To investigate the influence of DO concentration on the characteristics of catching bed reactor, the removal efficiency of COD, NH4+-N and TN were analyzed under the DO concentrations at 1.0~2.0mg/L, 2.0~3.0mg/L and 3.0~4.0mg/L, respectively. Meanwhile, ammonia-oxidizing bacteria (AOB) community and diversity in each reactor were examined using restricted fragment length polymorphism (RFLP), and sequencing of amoA genes. The results showed that higher COD and NH4+-N removal efficiency were received under different DO concentrations, NH4+-N removal efficiency increased with the DO concentration increase. The diversity of AOB was abundant in different DO concentrations, but the communities and species of AOB were effect greatly by DO conc