固相萃取结合高效液相制备茶树没食子酸衍生物.pdfVIP

茶叶科学2012，32(6)：494～499 JournalTeaScience of 固相萃取结合高效液相制备茶树没食子酸衍生物 卢忠尉1，蒋晓岚2，刘亚军1，刘莉2，高丽萍r，夏涛2+ 1．安徽农业大学生命科学学院，安徽合肥230036； 2．安徽农业大学教育部茶叶生物化学与生物技术重点实验室，安徽合肥230036 摘要：没食子酸衍生物是茶树酚酸中的主要成分，是茶汤中重要的鲜味增强剂，具有抗过敏、抗抑郁和增强 认知能力等作用。利用固相萃取(solid—phase high performanceliquid 纯化效果好。 关键词：没食子酸衍生物；固相萃取：制备高效液相色谱：13-D一没食子酰葡萄糖；3-O．没食子酰奎尼酸 中图分类号：TS272；Q946．8 文献标识码：A ofGallicAcidDerivatives Preparation by ExtractionC with High artridgeCoupledPreparative Performance fromTeaPlant Chromatography Liquid LU Xiao—lan2，LIU Li2，GAO Ta02+ Zhong．weil，JIANG Ya-junl，LIU Li—pin91+，XIA 1．Schoolof Science，Anhui Biology AgriculturalUniversity，Hefei230036，China； Labof and of Tea Biotechnology，MinistryEducation，AnhuiAgricultural 230036，China 2．Key Biochemistry University，Hefei arethechief of acidln are Abstract：Gallicacidderivatives compoundsphenolic tea．Theyimportant intea theeffectsof umami-enhancingcompoundsbeverage，andpossess inhibitingIgE PHPLC withSPEwas to andto coupled developed cognition—antingdepressant．Areversed—phase purify preparehigh acidand fromfreshtealeaves．Theobtainedfractionswere purity3-O—galloyquinicB—D—glucogalline successfully is identified and”C—NMR．Thismethod forthe of LC／TOF—ESI—MS，1H—NMR