有哪些信誉好的足球投注网站a novel method for the measurement of hepatitis c virus infectious titres using the incucyte zoom and its application to antiviral screening论文.pdfVIP
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Journal of Virological Methods 218 (2015) 59–65
Contents lists available at ScienceDirect
Journal of Virological Methods
j o u r n a l h o me p a g e : w w w. elsev /locate/jviromet
A novel method for the measurement of hepatitis C virus infectious
titres using the IncuCyte ZOOM and its application to antiviral
screening
Hazel Stewart a , Christopher Bartlett a , Douglas Ross-Thriepland a , Joseph Shaw b ,
Stephen Griffin b , Mark Harris a,∗
a School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom
b Leeds Institute of Cancer and Pathology, Faculty of Medicine and Health, St James’s University Hospital, University of Leeds, Leeds LS9 7TF, United Kingdom
s u m m a r y
Article history: Hepatitis C virus (HCV) is a significant human pathogen infecting 3% of the world population. An infec-
Received 1 December 2014 tious molecular clone capable of replicating and releasing infectious virions in cell culture has only been
Received in revised form 11 March 2015 available since 2005, leaving a significant knowledge gap concerning post-RNA replication events such
Accepted 12 March 2015
as particle assembly, trafficking and release. Thus, a fast, efficient and accurate method of measuring
Available online 20 March 2015
infectious viral titres is highly desirable. Current methods rely upon manual counting of infected cell foci
and so are both labour-intensive and susceptible
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