安非他明对小鼠白细胞介素10释放的影响及多巴胺D3受体的调控作用_临床医学论文.docVIP

安非他明对小鼠白细胞介素10释放的影响及多巴胺D3受体的调控作用_临床医学论文 安非他明对小鼠白细胞介素10释放的影响及多巴胺D3受体的调控作用_临床医学论文 作者：陈艳炯，朱俊艳，张洪波，魏曙光，阎春霞，郑海波，任会勋，陈腾 【摘要】 　　目的 研究安非他明(AM)对细菌脂多糖(LPS)刺激后小鼠白细胞介素释放的影响以及多巴胺D3受体在此过程中参与的调控作用。方法 实验Ⅰ：采用多巴胺D3受体基因敲除小鼠(D3RKO)以及具有相似遗传背景的野生型小鼠(C57BL/6J)，腹腔注射AM(5mg/kg)10min后再注射LPS(150μg/kg)刺激，在LPS给药后不同时间点(0、30、60、90、120、240min)心脏穿刺抽血进行IL测试，观察上述不同时间点D3RKO和C57BL/6J小鼠IL的分泌情况。实验Ⅱ：在实验Ⅰ所确定的小鼠IL分泌最高时间点，观察不同剂量AM (0、2、5、10mg/kg) 对D3RKO和C57BL/6J小鼠IL分泌的影响。结果 实验Ⅰ：AM 5mg/kg在LPS注射后可以引起D3RKO和C57BL/6J小鼠IL分泌增多，其中60min达最大值，与生理盐水对照组比较差异有统计学意义(0.05)。实验Ⅱ：C57BL/6J小鼠以AM 5mg/kg作用后IL增多最为显著，D3RKO小鼠以AM 2mg/kg作用后IL升高最为显著，与生理盐水对照组相比差异均有统计学意义(0.05)，且二者之间亦存在统计学差异。结论 AM对小鼠免疫功能的抑制可能是通过增加IL分泌而起作用；小剂量AM即可引起D3RKO小鼠血中IL升高，提示多巴胺D3受体可能参与了AM对小鼠免疫功能抑制作用的调控。 【关键词】 安非他明 白细胞介素 10 多巴胺D3受体 细菌脂多糖 基因敲除小鼠 　　ABSTRACT: Objective To study the effects of amphetamine (AM) administration on the production of interleukininvolved in it. Methods In section Ⅰ: dopamine D3 receptor knockout mice (D3RKO) and wild type mice (C57BL/6J), both exhibited a similar genetic background, received either vehicle(normal saline, NS) or AM (5mg/kg ip) 10min prior to LPS (150μg/kg) in vivo immune challenge, were sacrificed 0, 30, 60, 90,120, or 240min later by anesthetized with sodium pentobarbital; in sectionⅡ: C57BL/6J and D3RKO mice received AM at different dose (0, 2, 5, 10mg/kg) 10min prior to either vehicle or LPS, and were sacrificed 60min later. In anesthetized mice, blood was collected via cardiac puncture using a syringe without an anticoagulant. IL10 of serum was measured by means of commercially available enzymelinked immunosorbent assay. Results In section Ⅰ: under the treatment of AM, the greatest effect was observed at 60min after LPS challenge in both C57BL/6J and D3RKO mice. In sectionⅡ: the administration of AM at dose of 5mg/kg significantly increased LPSinduced IL10 production in C57BL/6J mice, but significantly increased LPS　induced ILd type, indicating that D3R mediates immune inhibition of AM. 　　KEY WORDS: amphetamine; interleu