080319生物信息学分析技巧.ppt

2000-01-20 1999年博士后中期考核报告 2000-01-18 1999年博士后中期考核报告 1999年博士后中期考核报告 数据库的相似序列有哪些信誉好的足球投注网站 军事医学科学院 放射与辐射医学研究所 李稚锋 2008.3.19 数据库相似性分析需求 确定新基因 特异引物和通用引物设计 新基因的功能研究 进化分析 内容提纲 基本知识 核酸相似性分析 编码序列 非编码序列 基因组 蛋白质相似性分析 序列 结构域 第一部分：基本知识 生物信息学 生物信息学(Bioinformatics) 是在数学、计算机科学和生命科学的基础上形成的一门新型的交叉学科，它是为理解各种数据的生物学意义，运用数学、计算机科学与生物学手段进行生物信息的收集、加工、存储、传播、分析与解析的科学 常用生物信息资源 NCBI / EBI http://www.ebi.ac.uk/ UCSC Genome Browser / TRANSFAC / http://www.gene-regulation.de/ KEGG http://www.genome.jp/kegg/ GO / DIP / NCBI / Databases at the EBI EMBL Nucleotide Database - Europe’s primary collection of nucleotide sequences is maintained in collaboration with Genbank (USA) and DDBJ (Japan) Ensembl - Providing up to date completed metazoic genomes and the best possible automatic annotation. UniProt – (Universal Protein Resource) is the worlds most comprehensive catalogue of information on proteins. It is a central repository of protein sequence and function created by joining the information contained in UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and PIR. The UniProt Knowledgebase (UniProtKB) The UniProt Reference Clusters (UniRef) The UniProt Archive (UniParc) The UniProt Metagenomic and Environmental Sequences (UniMES) InterPro - InterPro is a database of protein families, domains, repeats and sites in which identifiable features found in known proteins can be applied to new protein sequences. GOA - Gene Ontology Annotation Database. GOA has been a member of the GO Consortium since 2001. The GOA project aims to provide high-quality Gene Ontology (GO) annotations to proteins in the UniProt Knowledgebase (UniProtKB) and International Protein Index (IPI) and is a central dataset for other major multi-species databases; such as Ensembl and NCBI. Macromolecular Structure Database - European Project for the management and distribution of data on macromolecular structures ArrayExpress - for gene expression data IntAct - Provides