Plackett-Burman设计和均匀设计法优化葫芦巴碱的提取工艺.pdfVIP

第十届中国香料香精学术研讨会论文集 Plackett．Burman设计和均匀设计法优化葫芦 巴碱的提取工艺 肜霖黄龙谭烨董世良赵国豪 武汉黄鹤楼香精香料有限公司，武汉430040 【摘要】首先用Hacker．Burman设计对影响葫芦巴提取物得率的因素进行了评价，筛选出具 有显著影响的三个因素——乙醇浓度、溶剂用量和提取时间，然后用均匀设计法优化了主要影 响因素的最佳提取条件为乙醇浓度为90％，溶剂用量为4倍，回流提取4小时，在此条件下葫 结合均匀设计法可以减少实验次数，较好的对葫芦巴碱的提取工艺进行优化。 【关键词】葫芦巴碱；Hacker．Burman设计；均匀设计法；提取 trigonelline ofExtractionConditionsof from Optimization Trigonelline ‘ ● -uurmanana foenum PlackettPlackett．一D Triaonalla L．by IOenum-gracecumby Design Uniform Design RONG Lin，HUANG Guohao Long,尉ⅣYe，DONGShiliang,ZHA0 Wuhan Co．．Ltd．，Wuhan430040，China FlavorsFragrances Huan曲elou Abstract：Somefactors theextractionrateof were evaluated affecting trigonellinefirstly by Plackea-Burmanshowedthatthreefactorshave effects．Thentheextraction design，results significant conditionswere uniform resultsshowedthatthe extraction optimizedby design．The optimal conditionsareasfollows：ethanolconcentrationvolumewas4times．andt11eextraction 90％；solvent this extract was1 was time4h．Under conditions，the content yield 9．68％，trigonelline indic