麝香对颅骨骨缺损模型大鼠SDF-1表达的影响.pdfVIP

麝香对颅骨骨缺损模型大鼠SDF-1表达的影响 谢兴文1赵永利2李 宁2徐世红1姜 徽 2李盛华3宋 敏 2 1.甘肃省中医药研究院骨伤病研究所，7300502.甘肃中医学院，730003.甘肃省中医院730050 摘要：目的：观察不同剂量麝香对颅骨骨缺损模型大鼠骨缺损处SDF-1表达的影响，探讨麝香促进外源性rBMSCs 向骨缺损处迁移的机制。方法：采用贴壁筛选法培养rBMSCs，用流式细胞仪鉴定rBMSCs；大鼠颅骨骨缺损模型的建 立后回植rBMSCs；将模型组大鼠完全随机分为四组，高、中、低剂量组及空白对照组，并向高、中、低剂量组灌服麝 香，空白对照组灌服生理盐水；14d后处死大鼠取出缺损部位的颅骨，并对其脱钙、制作切片；免疫组化染色，光学显 微镜下观察，每个切片随机选取3个视野，进行统计学分析。结果：筛选纯化的rBMSCs呈均一的成纤维细胞样，贴壁 生长，以长梭形为主，漩涡状盘旋排列，表型鉴定结果为CD45阴性表达，CD44，C90表达阳性，rBMSCs经诱导后可 向成脂、成骨分化，麝香能促进颅骨骨缺损模型大鼠骨缺损处SDF-1表达，给药各浓度组和空白组比较有显著统计 学差异(P＜0.05)，以低浓度效果最佳。结论：麝香均促进外源性rBMSCs在大鼠体内向损伤部位迁移的机制与麝香促 进颅骨骨缺损模型大鼠骨缺损处SDF-1表达有关。 关键词：rBMSCs;麝香；SDF-1；免疫组化；迁移；机制 MuskskullbonedefectmodelofratsSDF-1theinfluenceofexpression XIEXing-wenlZHAOYong-li2LINing2XUShi-hong1JIANGHui2LISheng-hua3SONGMing21CansuProvinceAcademyof TCM,Lanzhou7300502GansuUniversityofTCM,Lanzhou730000 3GansuProvinceHospitalofTCM,Lanzhou730050China Abstract:Objective:ToobservetheinfluenceofdifferentdosesofmuskonSDF-1expression atthebonedefectoftheskullbone defectmoderat,toexplorethemechanismofthemuskpromoteexogenousrBMSCsmigratetothebonedefect.Methods:Using adherencescreening methodculturedrBMSCs,identifiedrBMSCsbyflowcytometry.ReplantedrBMSCsaftersettedskullbone defectratmodel.Modelratswererandomlydividedintofourgroupsashighdose,middledose,lowdosegroupandblankcontrol group.Andthethreedosegroupswerefedwithmuskandtheblankcontrolgroupwasfedwithsaline.14dayslater,killedratsand removedthedefectpartsofskull, anddecalcifiedtheskullandsliced;Observedunderopticalmicroscope with immunohistochemicalstaining,eachslicewasselectedrandomlythreevisualfieldsandstatisticallyanalyzed.Results:Screeningof purifiedtherBMSCsashomogeneousfibroblast-like,adherentgrowth,longspindle,swirlingcirclingarrangementandphenotypic identifiedwithnegativeexpressioninCD45andpositiveexpressioninCD44andC90.TherB