麝香酮含药血清对大鼠骨髓间充质干细胞的增殖、分化的影向.pdfVIP

麝香酮含药血清对大鼠骨髓间 充质干细胞的增殖、分化的影向 谢兴文1侯费祎2李盛华1宋 敏 2徐世红1 1.甘肃省中医药研究院，7300502.，甘肃中医学院，730000 摘要：目的：研究麝香酮含1药血清对骨髓间充质干细胞的增殖及其向成骨细胞分化的影响，确定单体药物对骨 髓间充质干细胞增殖分化是否有影响。初步了解麝香酮单体对骨髓间充质干细胞作用机制。方法：贴壁筛选提取大 鼠BMSCs，培养至第三代，流式细胞仪检测细胞表面抗原，成骨成脂诱导，鉴定认为培养成功后，利用不同浓度麝香 酮含药血清干预。测定细胞增殖率、ALP活性、钙盐沉积量、骨钙素分泌量等，研究麝香酮含药血清对大鼠干细胞增 殖、分化的影响；结果：筛选纯化的BMSCs呈均一的成纤维细胞样，贴壁生长，以长梭形为主；表型鉴定：CD45阴性， CD44、CD95均呈阳性表达；用含药血清干预，测定增殖率、ALP活性、钙盐沉积量、骨钙素分泌量、钙化结节量均高 于空白对照组。结论：麝香酮含药血清可以促进骨髓间充质干细胞的增殖、成骨分化。 关键字：骨髓间充质干细胞；增殖分化；麝香酮；含药血清 Theimpactofmusconecontainingserumonratmesenchymalstemcellsproliferationanddifferentiation Abstrsct:Objective:Tostudytheimpactofmusconecontainingserumonmesenchymalstemcellsproliferationandosteogenic differentiation,todeterminewhetheritwillaffectthatthemonomerofdrugsonmesenchymalstemcellsproliferationand differentiation.Preliminarylearnthemechanismofmusconemonomertomesenchymalstemcells.Methods:adherencescreening extractedfromratBMSCs,andtrainingtothirdgeneration,thendetectingitssurfaceantigen,osteogenicandadipogenicinducedby flowcytometry,usingdifferentconcentrationsofmusconecontainingserumtointerventionWhenthatwereidentifiedsuccessfully cultured.Determinationoftherateofcellproliferation,ALPactivity,calciumdeposition,oeteocalcinsecretion,studytheimpactof musconecontainingserumonratstemcellproliferationanddifferentiation.Results:screeningofpurifiedBMSCsshowed homogeneousfibroblast-like,adherentgrowth,withlongfusiformmainly.NegativeCD45,CD44,CD90werepositive.Using containingserumintervention,determinationofproliferationrate,ALPactivity,calciumdeposition,osteocalcinsecretion,calcified noduleswerehigherthantheblankcontrolgroup。 Conclusion:musconecontainingserumcanpromotethemesenchymalstemcellsproliferation,osteogenicdifferentiation. Keywords:mesenchymalstemcells;proliferationanddifferentiation;muscone;containingserum 中华中医药学会骨伤科分会ij312年学带年会燃·垂础部分 体外培养方法简单，扩增容易，可分化为各种中胚层组