花生基因转化体系的优化的研究.pdfVIP

Inter口afionalC0n研即∞OnPlant 2005植物分子育种国际学术研讨会论文集 proceedingsofnhe2005 MolecularBreeding,27—30，Oct．．2005，SanyqChina 2005年lO月27-30[了，中国，三亚 on Efficientof L．)Ge— Peanut(Arachis StudyHigh System hypogaea neticTransformation Liu FengzhcnWan YongshanWangHonggang CollegeofAgronomy,ShandongAgriculttwa]University,Taian,271018 coin ‘Colxespoadingauthcn,Ifz@tal39 of Inthis wasdoneontheeffectof condition Abstractexperiment，systematicstudy physiology explants； and on ofthe cultivarswereusedas medium genotype componentsplantregenerationtransgenicplants．6peanut was efficient source established．Hiigh ofexplants．111eh诎efficientsystemofpeanutplantregeneration system transformationwag ofsome factors flails- established ofpeanutgenetic byanalysis influencingofpeanutgenetic fommtionin orderto withtheresistance fun· procedures．In transgenicplantsofpeanut ofpeanut many generate wasintroducedintothree culfivars． gusdisease，Rs—q廓lgene peanut Tissue PeanuLculture，Genetic proteingene, Keywords transformation,Raphnaussativus—antifungal 花生基因转化体系的优化研究 刘风珍‘万勇善王洪刚 山东农业大学农学院．泰安，271018 tom 通讯作者．tfz@tal39 摘 研究了外植体类型、生理状态、基因型、培养基激素等因素对花生离体培养再生的影响，明确了器官发生途 径的技术条件和再生效率，建立了高效的花生叶片离体培养植株再生体系。研究优化了农杆菌介导途径进 行花生遗传转化的技术要素，建立了高技的遗传转化体系。利用这一体系，把抗真菌的1一硫堇蛋白哝s— a帛1)基