垃圾降解过程中淀粉酶活性测定条件优化.pdfVIP

垃圾降解过程中淀粉酶活性测定条件优化* # 廖 禧 邱忠平 童 菲 周丽萍 (, 610031) , , , 6 (L ( 5 ) ) : ( 1) , 25 ( 2) 30 , 7 mL 1%( ) 10 mL pH 5. 6 0. 2 mL , 35 36 h( 3) , ( RSD) 1. 96%, ; 97. 9%, RSD 1.75%, Optimal analytical procedure for measuring amylase of solid waste degradation L IA O X i, QI U Zhongp ing , T ON G Fei, ZH O ULip ing . ( School of L if e Science and Eng ineering , S o thw est Jiaotong University, Chengd S ich an 610031) Abstract: mylase is a key enzyme of the carbon conversion processes in landfill. Using the orthogonal experi 6 mental program ( L (5 ) ) and based on soil enzymology, the analytical procedure for measuring amylase in landfill 25 was optimized for the landfill waste sample. T he optimal procedure was reliable and precise; the amylase in landfill measured under the optimal experimental conditions (30 of dry temperature,7 mL of 1% starch solution, 0. 2 mL of toluene, 10 mL of pH 5. 6 phosphate buffer solution and 35 of culturing for 36 h) had a small relative standard de viation ( RSD) of 1. 96%. Keywords: waste degradation; amylase; orthogonal test; optimal conditions [7] , , ,, , , [ 1, 2] , , [3,4] [ 8] , , ( , [5] , ) 5~ 7 , 15 d 20% 1 [6] ( ,) 7% , , 1. 1 样品来源 , 1.2 实验原理 , , , , , # : , , 1984 , ,