川芎嗪聚乳酸纳米粒的包封率测定.pdfVIP

2011 1 2 1 OURNA L OF NAN ING TCM UNI VERSI T Y Vol. 2 N o. 1 an. 2011 ∀ ∀ * 1 1 2* 1 李争艳 , 毛春芹 , 曾莉 , 陆兔林 ( 1. , 210046; 2. , 210046) : 建立川芎嗪聚乳酸纳米粒包封率测定方法 采用Hypersil ODS2( 250 mm 4. 6 mm, 5m) 谱柱, 流动相为甲醇- 水(52 48) , 流速为1 mL/ min, 柱温: 30 ! , 检测波长为 2 9 nm 的 谱条件, 低温超速离心法测定包封 率 该方法可以用于测定川芎嗪聚乳酸纳米粒的包封率 : 川芎嗪; 聚乳酸; 纳米粒; 包封率 : R283. 6 : A : 1000- 5005(2011) 01- 00 - 03 Determination of EntrapmentEfficiencyof TetramethylpyrazinePolylactic AciNanoparticles * LI Zhengy a n, MAO Chunqin, ZENG L i ,L U Tulin (1.S chool of Pharmacy of N anj ing University of Chinese M ed icine, N anj ing , 210046,China; 2 .N anj ing University of Chinese M edicine L ibrary, N anj ing , 210046,China) ABSTRACT:OBJECTIVE To develop a method for det ermining the entrapment efficiency of tetramethylpyrazine polylac tic aci nanopart icles. ETHODS T he determining was performed by H PLC wit h Hypersil ODS2( 250mm 4. 6mm, 5m) conlumn at 30! . The mobile phase was methanolwater( 52: 48) , the flow rate 1. 0 ml/ min and the UV det ection wavelength 2 9 nm. U ltracentrifugation method w ith low t em perat ure was used to determine the ent rapment efficiency of tetramethylpyrazine polylactic aci nanoparticles. CONCLUSION T he designed method can be used for determining the en trapment efficiency of tetramethylpyrazine polylactic aci nanopart icles. KEY WORDS:tetramethylpyrazine; polylactic aci; nanopart icles; entrapment efficiency , 99% , , [ 1] 90% , ) , ( MW= 20 000, ) [ 2]