几种外部因子对春石斛组培生根的影响.pdfVIP

安徽农业科学 , Journal of Anhu i A gri. Sci. 2008, 36 (29) : 1259 1 - 12592 , 12594 责任编辑 　庆瑢 　责任校对 　张士敏 几种外部因子对春石斛组培生根的影响 ( ) 潘虹虹 ,孙 丹 ,廉美兰 ,朴炫春 　 延边大学农学院园艺系 ,吉林龙井 133400 摘要　[ 目的 ]为春石斛种苗的规模化生产提供理论依据 。[方法 ]以春石斛组培增殖的不定芽为外植体 ,研究 M S培养基浓度、IBA 浓 度 、活性炭浓度及天然物质等外部因子对春石斛试管苗生根的影响。[结果 ] M S培养基浓度对试管苗生根影响较大 ,试管苗在 1/2 M S 培养基中生根效果较好 ,生根率达 95% ,根多且长 。在 1/ 2 M S培养基中加入 0. 5 m g/L IBA 和 0. 5 m g/L 活性炭对试管苗生根及生长有 明显促进作用 。在培养基中添加天然物质对试管苗生根有促进作用 ,其中添加 100 g/L 椰子汁时生根效果最佳 ,生根率可达 97% 。[结 论 ]当培养基配方为 1/2 M S + 0. 5 m g/L IBA + 0. 5 m g/L 活性炭 + 100 g/L 椰子汁时,春石斛试管苗生根及生长效果较好 。 关键词　春石斛;培养基浓度 ;活性炭;天然物质 ( ) 中图分类号　S682. 31　　文献标识码　A　　文章编号　0517 - 6611 2008 29 - 12591 - 02 Effect of Severa l Externa l Factor s on Rooting of T issue Culture Seedling of D en drob ium n ob ile ( ) PA N Honghong et a l　 Horticu lture D ep artm ent, College of A gronomy Yanb ian Un iversity, Longj ing, J ilin 133400 A b stract　[Objective ] The study was to p rovide the theoretical basis for scale p roduction ofD end robium nobile seedlings. [M ethod ] W ith ad ventitiou s bud s p ro liferated through tissue cu lture of D. nobile a s exp lants, the effects of several external factors such a s the concn. ofM S m e dium , IBA , activated carbon and natural sub stance etc on rooting of tube seedlings of D. nobile were studied. [ R e su lt ] The effect ofM S m e dium concn. on rooting of tube seedling wa s b igger, and the rooting effect of tube seedling in 1 /2 M S m edium was better, the rooting rate reached 95% , and the roots were more and long. It had obviou s p romoting effect on rooting and grow th of t