AFLP和RFLP标记检测水稻亲本遗传多样性比较研究陈亮.pdfVIP

　2002, 35 (6):589-595 Scientia Agricultura Sinica AFLPRFLP 1 2 1 2 1 2 1 陈　亮,梁春阳, 孙传清, 金德敏 ,姜廷波, 王　斌, 王象坤 1 2 (, 　100094;, 　100101) :利用AF LP 和RF LP 技术对20 个水稻品种(系)进 了遗传多样性分析。在AF LP 分析中, 用64 对引物组 合进 了初筛, 然后用其中适合水稻的15 对引物进 了详细研究, 每对A FLP 引物组合扩增出了47 ～118 条带, 其中 多态性带为5～32 条, 15 个引物组合共得到多态性条带299 条;在RF LP 分析中,49 个RF LP 探针共检测出107 条多 态性带。聚类分析表明, 两种标记均能将20 个材料分成籼粳两大类群, 但AF LP 标记比 RFLP 标记更能反映品种间 系谱亲缘和地理生态上的差异。 两种标记检测出的遗传距离的趋势是 一致的, 但AF LP 标记相对于RF LP 而言提高 了品种间的遗传距离的估计, 降低了亚种间遗传距离的估计, 因此,A FLP 更适应于多样性的研究, RF LP 更适应于籼 粳分类及度量籼粳分化程度。 :水稻;A FLP;RF LP;聚类分析;遗传多样性 Comparison Between AFLP and RFLP Markers in Detecting the Diversity of Rice (Oryza sati va L.) 1 2 1 2 CHEN Liang , LIANG Chun-yang , SUN Chuan-qing , IN De-min , 1 2 1 IANG T ing-bo , WANG Bin , WANG Xiang-kun 1 (Department of P lant Genetics and B reeding, China Ag ricultural University , B eij ing 100094 ; 2 nstitute of Genetics, Chinese A cademy of Sciences, Beij ing 100101 ) Abstract:The AFLP and RFLP were used to study the diversity of 20 rice varieties.The 15 primer combi- nations were used in the analysis of AFLP and 47-118 bands w ere am plified in each lane.A total of 107 poly- morphic bands were detected in the RFLP analysis using 49 RFLP probes.The cluster analysis based on RFLP data showed that 20 rice varieties could be divided into indica group and japonica group, but the AFLP is more suitable in detecting the difference of pedigree and ecoty pe among the