两次诱导实现重组大肠杆菌高密度_高表达研究.pdfVIP

# 54# 2005 32 ( 2) * * * 马文峰 庄英萍 郭美锦 丁满生 储 炬 张嗣良 ( 200237) : , , -1 , OD 600 150, 41 8g# L , , : , ApoA-I , , , M ilano : TQ92016 : A : 0253-2654 ( 2005) 02-0054-06 Study on the Recomb inant Hum an ApoA- I H igh D en sity and H igh M ilano * Expression by Two T emperature-Sh ifted Induction in Escherichia co li * * MA W n-F ng ZHUANG Y ing-Ping GUO M i-Jin DINGM an-Sh ng CHU Ju ZHANG S-i Liang (East Ch ina Un iversity of S cience and Technology, S tateK ey La boratory of B ioreactor E ng ineering, Na tional R eserch Cen terf or B iotechnology, Shanghai 200237 A bstract Th tm p ratur ff ct on th r combinant prot in production form ation w as inv stigat d in pr s nt study1 Th cultur t mp ratur of grow th phas is 30e , and th cultur t mp ratur of induction phas w as ar- rang d according to thr mod s1H ign c ll-d nsity and high xpr ssion cultur ofE 1coli to product r combinanthu- m an apo lipoprot in A-I by wt o t mp