从水稻种胚中提取RNA的新方法.pdfVIP

遗 　传 HER EDI TA S ( Beijin g) 25 (2) :208～210 ,2003 　　 研究报告 从水稻种胚中提取 RNA 的新方法 沈文飚 ,汪　仁 ,王益华 ,郑天清 ,万建民 (南京农业大学作物遗传与种质创新国家重点实验室 ,南京农业大学水稻研究所 ,南京卫岗 210095) 摘 　要 :介绍了一种全新、简单的提取水稻种胚 RNA 的 CTAB - LiCl 提取法 ,可在不用液氮的室温下有效地排除剥 取的种胚中大量带有的多糖和本身脂质的干扰 ,所得 RNA 样品经紫外分光光度计和琼脂糖凝胶电泳分析证明具 有较高的纯度和完整性 ,并可进一步满足 RT - PCR 和 Northern 印迹的实验需要 ,适用于富含多糖和脂质的植物组 织 RNA 的提取。 关键词 :水稻种胚 ;RNA ;CTAB - LiCl 提取法 ;多糖 ;脂质 ( ) 中图分类号:Q75 　　　文献标识码 :A 　　　 文章编号 :0253 - 9772 2003 02 - 208 - 03 A Novel Method of Extracting Total RNA from Rice Embryo Samples SHEN WenBiao ,Wang Ren ,WAN G YiHua ,ZHEN G TianQing ,WAN JianMin ( State Key L aboratory of Crop Genetics and Germplasm Enhancement , Rice Research Instit ute , N anji n g A gricult ural U niversity , N anji n g 210095 , Chi na) Abstract :A novel and simple CTAB - LiCl - based extraction method for high - quality and total RNA of rice embryo samples is developed. This method can efficiently eliminate the interference of polysaccharide and lipids rich in rice ( Oryz a sativa L . ) embryo obtained under room temperature without using liquid nitrogen. The results of ultraviolet spectropho tometer and agarose gel electrophoresis analysis show that the obtained RNA has no obvious degradation and a good purity sufficient for further RT - PCR and RNA gel blotting. Therefore ,it is also especially useful for the RNA extraction of plant material plenty of polysaccharide and lipids. Key words :rice ( Oryz a s