GC_MS结合直观推导式演进特征投影法_HELP_用于桂花挥发油成分的分析.pdfVIP

※分析检测 食品科学 2009, Vol. 30, No. 24 28 7 GC-MS结合直观推导式演进特征投影法 (HELP)用于桂花挥发油成分的分析 1,2 1, 1 1 1 胡春弟 ，梁逸曾 * ，李小如 ，郭方遒 ，曾茂茂 ( 1.中南大学化学化工学院中药现代化研究中心，湖南长沙 410083 ；2 .咸宁学院药学院，湖北咸宁 437100) 摘 要：通过气相色谱- 质谱联用(GC-MS)结合直观推导式演进特征投影法(HELP) ，对桂花挥发油成分进行定性和 定量分析，鉴定出α- 甲基- α-(4 - 甲基-3 - 戊烯基) 环氧甲醇、β- 里哪醇、丁香醛、(E )- β- 紫罗兰酮、棕榈酸、 水杨酸甲酯等48 种组分，占出峰总面积的92.79% ，主要成分为α- 甲基- α-(4 - 甲基-3- 戊烯基)环氧甲醇、4-(2,6,6- 三甲基环己- 1- 烯) 丁-2 - 醇、5- 乙烯基四氢- α, α,5- 三甲基-2 - 呋喃甲醇，三者总相对含量达62 .32 % 。结果表明， 使用H EL P 法对重叠峰进行解析，得到纯的色谱和质谱，从而大大提高了分析结果的准确性。 关键词：G C -M S ；直观推导式演进特征投影法；桂花；挥发油 Essential Oil Composition of Osmanthusf ragrans by GC-MS Combined with Heuristic Evolving Latent Proj ection 1,2 1, 1 1 1 HU Chun-di ，LIANG Yi-zeng * ，LI Xiao-ru ，GUO F ang -qiu ，ZENG Mao-mao (1. Research Center of Modernization of Chinese Herbal Medicine, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China；2. School of Pharmacy, Xianning University , Xianning 437100, China) Abstract ：The essential oil composition of Osmanthus f ragrans was analyzed qualitatively and quantitatively by gas chromatog- raphy-mass spectrometry(GC-MS) under the help of heuristic evolving latent proj ection(HELP). 48 components were identified accounting for 92.79% of th e total area of peaks and the m ain compound s were α-methyl- α-[4-methyl-3-pentenyl] oxiranemethanol, β-linalool, lilac aldehyde, (E)- β-ionone, hexadecanoic acid, methyl salicylate contributing to 62.32% of total essential oil. T