宁强矮马生长激素基因(GH)的遗传变异分析.pdfVIP

中国农业科学 2009,42(12):4435-4440 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2009.12.040 宁强矮马生长激素基因（GH）的遗传变异分析 张 涛，路宏朝，陈 锐，周建军 （陕西理工学院生物科学与工程学院，陕西汉中 723001 ） 摘要：【目的】通过分析不同动物和中国几个地方马种生长激素基因序列，为宁强矮马起源和矮化形成机制 的研究提供分子水平的试验依据。【方法】通过 PCR 扩增宁强矮马生长激素基因并进行测序，用 DNAMAN 软件对不 同动物的生长激素基因序列进行比对分析。【结果】马品种间29个位点发生遗传变异，编码区11个位点发现遗传 多态现象。以蒙古马生长激素氨基酸序列为标准，宁强矮马仅在 1 765 位点的变异导致氨基酸发生改变。以生长 激素的mRNA序列为基本数据进行聚类分析，宁强矮马和德保马67的同源性为100%，与百色马的同源性为99.8%， 与蒙古马的同源性为 99.7%。【结论】马属动物生长基因序列与猪和狗的同源性较高，宁强矮马 1 765 位的 G→A 可能影响到宁强矮马的生长发育。 关键词：宁强矮马；生长激素基因；序列分析；遗传变异 Genetic Variation of Growth Hormone Gene on Ningqiang Pony ZHANG Tao, LU Hong-zhao, CHEN Rui, ZHOU Jian-jun (School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, Shaanxi) Abstract: 【Objective 】 In order to provide a scientific basis for the origin and dwarfish mechanism of Ningqiang pony, the sequence of GH of different animals and some China horse breeds were studied. 【Method 】 The PCR amplification, sequencing and DNAMAN software were used. 【Result 】 There were 29 genetic variation, 11 loci were found in the coding region. Taking the AA sequence of GH of Mongolia horse as standard, there was 1 amino acid variation in Ningqiang pony. Through clustering analysis based on the sequence of mRNA of GH, the homology was 100%, 99.8%, 99.7%, between Ningqiang and Debao 67, Baise and Mongolia. 【Conclusion 】 The homology level of GH was higher among Equus caballus, Sus scrofa and dog in present study, and the mutation of G→A affects the growth of Ningqiang pony. Key words: Ningqiang pony; growth hormone gene; sequence analysis; genetic variation 矮马起源进化和矮化机制提供试验依据。【前人研究 0 引言