应用依赖解旋酶DNA恒温扩增技术检测沙门菌的研究.pdfVIP

2010 12 20 12 Chi ese Jour al ofH ealth Laboratory Tech o logy, Dec 2010; Vo l20 N o 12 3289 应用依赖解旋酶DNA恒温扩增技术检测沙门菌的研究 1, 2 2 2 1 2 2 王建广 , 姜英辉 , 雷质文, 石琰璟 , 房保海, 刘云国 ( 1. , 266042; 2. , 266002) [ ] : DNA (H elicase- depe de t Isotherm al DNA Am plificatio , HDA ), (Sa lm onella ): i vA , HDA , , , PCR : HDA , 460 pg/ tube, PCR: HDA , , [ ] 沙门菌; 检测;依赖解旋酶 DNA 恒温扩增 术 + [ ] R378. 2 2 [] A [] 1004- 8685( 2010) 12- 3289- 03 Study on detection ofSa ml onella withHelicase- dependent isothermalDNA amplification technology 1, 2 2 2 1 2 2 WANG J ian - guang , JIAN G Ying - hui , LE I Zhi- w en , SH I Yan- j ing , FAN G B ao - ha i , LIU Yun - g uo ( 1. Q i gdao U iversity of Scie ceTech ology, Q i gdao 266042, Chi a; 2. Sha do g E try- Exit I spectio a d Quara ti e Bu reau, Q i gdao 266002, Chi a) [Abstract] Objective:To establish a ew rap id m ethod to detectSalmonella of samp le wh ich based o H elicase- depe de t i sotherm al DNA am plificatio ( HDA ). ethods: A high ly specific set of prmi ersw ere sy thesized to target the i vA ge e ofSal m onella. Specific a d se sitivity was tested wh ich w as com pared w ith PCR m ethod. Results: H elicase- depe de t isotherm al DNA amp lificatio m ethod that ca rap id ly detect Salm onella has bee established. The se sitivity was 460 pg/ tube wh ich w as smi ilar to the result of PCR method. Conclusion: Detecti g Salm onella w ith HDA is a rap id, user- frie d ly , specific, se sitive m ethod. It!s very su itab le for prmi ary laboratory use, w ith a b road prospect. [Keywords] Salmonella;