绿原酸的分离纯化.pdfVIP

※工艺技术 食品科学 2007, Vol. 28, No. 07 255 杨敏丽，郝凤霞 (宁夏大学能源化工重点实验室，宁夏 银川 750021) ：目的：对金银花中的绿原酸的提取、分离工艺进行研究。方法：绿原酸经乙醇热回流提取后，采用 聚酰胺柱层析法进行分离除杂，对主要工艺参数进行了优化。结果：将待分离的绿原酸粗品和聚酰胺(80～100目) 按物料比1:5装入柱径与柱长比为1:7的层析柱中，常压下用10%的乙醇以最大流速洗脱至14倍柱体积，收集绿原 酸含量较高(A322/A216 ≥ 0.9)的流分，浓缩后得到纯度为70% 的绿原酸产品，经重结晶后纯度可提高到93%。结 论：该工艺简单、成本较低，适于工业化。 ：金银花；绿原酸；聚酰胺柱层析；分离；纯化 Study on Isolation and Purification of Chlorogenic Acid in Flos Lonicerae YANG Min-li, HAO Feng-xia (Key Laboratory of Energy Sources Chemical Engineering, Ningxia University, Yinchuan 750021, China) Abstract ：Objecive: To study the isolation and purification conditions of chlorogenic acid(CA) from Flos Lonicerae. Method: The isolation and purification of CA were achieved by polyamide column chromatography and the main isolation factors were optimized. Results: The optimum isolation is as following: the rate of crude CA to polymide is 1:5, the rate of diameter to long is 1:7, 10% alcohol washed at high speed under usual pressure, CA is collected between the volume of 5 ～ 14 times of column volume(A322/A216≥0.9), under the conditions, the product with above 70% CA is obtained, which can be further crystalled by EtoAc and the purity is enhanced to 93%. Conclusion: This method is simple, low cost, and suitable to be used in industry. Flos Lonicerae；chlorogenic acid；polyamide column chromatography；isolation；purification Key words： 中图分类号：Q946.8 文献标识码：A 文章编号：1002-6630(2007)07-0255-05 金银花属忍冬科植物忍冬Lonicera japonica Thunb 层析对粗提物进行分离除杂，并对影响分离的主要工艺 的干燥花蕾或初开的花，是传统的清热解毒常用中 参数进行了优化选