核黄素基因工程菌的构建及其发酵的初步研究.pdfVIP

21 2 No.2 Vol.21 2007 4 Journal of Chemical Engineering of Chinese Universities Apr . 2007 1003-9015(2007)02-0356-05  , , , , , ( , 300072) pRB63 B.subtilis B.subtilis RH13 RH13::[pRB63] pRB63 n RH136~7RH13::[pRB63] B.subtilis YB1 n B.subtilis RH33 10 64 h 4.2 g⋅L− 1 24 h 7~8 g⋅L− 148 h 11~12 g⋅L− 10.056 g⋅g− 1 TQ466.2 Q563.2 Q813.2 A Construction of Genetic Engineered Bacillus subtilis for Riboflavin Production in Fed-batch Fermentation CHEN Tao, DONG Wen-ming, LI Xiao-jing, WU Qiu-li, CHEN Xun, ZHAO Xue-ming (Department of Biochemical Engineering, School of Chemical Engineering Technology, Tianjin University, Tianjin 300072, China) Abstract: An integration plasmid for Bacillus subtilis, pRB63, which contains a deregulated riboflavin operon of Bacillus subtilis was constructed and then transformed into B.subtilis RH13 which is a riboflavin producing strain. Engineered strains RH13::[pRB63]n were obtained by gene amplification of the integration plasmid on the host chromosome. The gene amplification results in an enhancement of riboflavin productivity which is 6-fold higher than the corresponding values of RH13 in batch fermentation. Subsequently, B.subtilis RH33 was screened by protoplast fusion of B.subtilis RH13::[pRB63]n and another riboflavin producing