不同基因型刺梨及其近缘种亲缘关系的RAPD分析.pdfVIP

农业生物技术学报 Journal of Agricultural Biotechnology 2003,11 6冤院 605~6 11 ·研究论文· Characterization of Genotypes and Genetic Relationship of Cili ( ) and Its Relatives Using RAPD Markers* Wen Xiaopeng1,2 Deng Xiuxin 1** (1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; 2. Agricultural College, Guizhou University, Guiyang 550025, China) Cili ( Tratt) ，characterized by containing the highest content of vitamin C among fruits and showing attractive senescence-retarding and cancer-preventing effects, has brought about statewide interest. RAPD markers were applied to identify the 7 genotypes of cili and to evaluate the genetic relationship within cili, as well as among its relatives. Sixteen arbitrary primers screened from 154 were adopted to analyze polymorphism in RAPD profiles for the 15 samples. A total of 137 RAPD bands ranging in size from 480 bp to 3.3 kb were obtained, among which 95 were polymorphic, covering 69.3% of the total bands obtained; And an average of 8.6 bands/primer was scored. The genotypes of cili and seedless cili could be efficiently identified by 14 genotype-specific bands which were obtained from the polymorphic primers OPB-11, OPAF- 16 and OPW-02. Additionally, a dendrogram of UPGMA showing genetic relationship of 15 samples was constructed based on cluster analysis of genetic distance and the genetic relationship between the samples. The possible origin of seedless cili and multiple-corolla cili were disc