两种真菌对紫茎泽兰的致病性研究.pdfVIP

天然产物研究与开发 NATURAI．P】瑚UCrAND REsl￡ARCHDEVELOPM哐NT 两种真菌对紫茎泽兰的致病性研究 向 明 廖薇侯若彤杨志荣* (四川大学生命科学学院成都610064) 摘要本文以紫茎泽兰离体叶片为材料，研究了从空气中分离得到的青霉属和曲霉属的两种真菌对紫茎 泽兰的致病性和叶片细胞膜透性的影响。结果表明，两种真菌及其毒素均能在紫茎泽兰叶片上产生直径2～ 5ITlrn的局部坏死斑。叶片用毒素处理后，细胞膜透性增大，相对电导率分别可达86．25％、94．56％；K+和 Na+渗漏量增大，且随着时间的延长和毒素浓度的增加而增高。 、 关键词紫茎泽兰；粗毒素；生物测定；致病机理 THEPATHOGENICITYOFTOXINFROMTWoFUNGI D!E2N℃)PHoR【月Ⅵ oNE【腥tA田0RI【月坦A XIANG Wei，HOU Ming，LIAO Ruo-tong，YANGZhi—rong。 Sichuan Departmentof University，Chengdu610064，China) (L咖Science AbstractToxins two isolatedfromnaturecouldincreasethemembrane by fungi produced incellof freshleafinvitro．We theculture permeabilityEupatoriumadenophorum prepared filtrateandcoarsetoxinofthesetwo infectthefleshleaves showed fungi，and separately．Results thattoxin influencethe lesiononleafwhichdiameterwasto could Eupatoriumadenophorum up --5 is differencedfromtheinfluencewater．Therelative of mm，which by conductivity obviously leavesworkedthesetwotoxinscouldincreaseto86．25 centand94．56 cent．Therewas by per per a correlationbetweenthe of ofK+andNa+andtheconcentrationof positive increasingleakage toxin． words adenophorum；coarsetoxin；bioassay；nosogenesis Key Eupatorium