surface display公告事项-Surface Display.ppt

Surface Display Phage Bacteria Yeast Ribosome Phage Display Three rounds of panning againststreptavidin at constant stringency (0.5% Tween)  His-Pro-Gln (HPQ) as a consensus binding motif specific for thebiotin binding site of streptavidin Capture of the antibody by Protein A-agrose Protein A is a cell wall protein deriving from Staphylococcus aureus which exhibits unique binding properties for IgG from a variety of mammalian species and for some IgM and IgA as well. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. Cell surface display Gram(+) cell Yeast Surface Display Screening Yeast Displayed Libraries Phage vs Ribosome Display Ribosome display * Displayed: passenger protein Anchoring motif: carrier protein Panning Filamentous phages are flexible rods about 1 mm long and 6 nm in diameter, composed mainly (87% by mass) of a tube of helically arranged molecules of the 50-residue major coat protein pVIII22; there are 2700 copies in wild-type virions, encoded by a single phage gene VIII. Inside this tube lies the single-stranded viral DNA (ssDNA; 6407-8 nucleotides in wild-type strains). At one tip of the particle there are five copies each of the minor coat proteins pIII and pVI (genes III and VI, respectively); minor coat proteins pVII and pIX (genes VII and IX) are at the other tip. Epitope mapping of the anti-FLAG M2 monoclonal antibody. In order to determine which elements of the reported FLAG epitope sequence Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (DYKDDDDK) are required for antibody binding Epitope mapping of an anti-β-endorphin monoclonal antibody with the Ph.D.-12 library Gram (-) cell Ice nucleation factor promoting ice forming Ice Nucleation Protein (INP) OmpC Staphylococall Protein A Phage display :(purple) ribosome display: (green) *