多种菌混合发酵玉米秸秆.pdfVIP

2011 No．6 · · Serial No．231 China Brewing 110 Research Report 多菌种混合发酵玉米秸秆生产微生物油脂的研究 殷 桃，李新社*，胡墩柱，何淑华，张 峰 （邵阳学院生物与化学工程系，湖南 邵阳 422000） 摘 要：利用绿色木霉、烟曲霉与皮状丝孢酵母共酵玉米秸秆，采用正交试验进行微生物油脂发酵。结果表明，多菌种混合发酵玉米 秸秆生产微生物油脂的最佳条件：绿色木霉、烟曲霉与皮状丝孢酵母配比为1∶1∶1，接种量为10%，培养温度为28℃，培养时间为7d。在 最佳条件下每100g玉米秸秆发酵产物中可获得1.247g油脂。研究表明，利用多菌种发酵玉米秸秆生产微生物油脂具有一定的可行性。 关 键 词：绿色木霉；烟曲霉；皮状丝孢酵母；玉米秸秆；微生物油脂 中图分类号：Q939.97 文献标识码：A 文章编号：0254-5071 （2011）06-0110-03 Production of microbial oils fermented by multi-strains with corn stalks YIN Tao, LI Xinshe*, HU Dunzhu, HE Shuhua, ZHANG Feng (Dep artment of Biology and Chemistry Engineering, Shaoyang University, Shaoyang 422000, China) Abstract: The corn stalks was co-fermented by Trichoderma viride, Aspergillus fumigatus and Trichosporon utaneum to produce microbial oil. The 4 optimal process conditions were determined by L （3 ）orthogonal test. The microbial oils content of the corn stalks co-fermented by T. viride, A. fu- 9 migatus and T. utaneum with the ratio of strains was 1 ∶1∶1, the inoculum of strains was 10% at 28℃ for 7 d was 1.247g /100g fermentation products. The research showed that there was certain feasibility on production of microbial oil fermented by multi-stains with corn stalks. Key words: Trichoderma viride; Aspergillus fumigatus; Trichosporon cutaneum; corn stalks; microbial oil 能源是经济社会发展的重要动力。面对全球社会经 原料成本的影响，工业化生产程度低，经济可行性差。因 济的迅速发展，人类对能源的需求日益增长，仅中国能源 此寻找一种廉价的原料成为了生物柴油产业化的关键。 [1]