中国穿山甲与爪哇穿山甲甲片异速生长分析及其在司法鉴定中的应用.pdf

3 期 周昭敏等：中国穿山甲与爪哇穿山甲甲片异速生长分析及其在司法鉴定中的应用 62 为了进一步确认输卵管在冬眠前期上皮与基质细胞的增殖与分化，对输卵管组织进行了 PCNA 的免疫印迹和免疫组织化学检测的实验。PCNA 的阳性表达显示出中国林蛙输卵管中上 皮与基质细胞的分裂活性发生于冬眠前期。 统和以上结果，包括 c-kit 受体蛋白的内源性因子在中国林蛙输卵管细胞的增殖与分化 过程中发挥调控效应。未来的研究需要从性类固醇激素的水平以及 c-kit 受体蛋白在冬眠前 期和冬眠后期的变化模式进行更深入地研究。 参考文献 [1]. Winter, W. E. , Seidman, J. D., Krivak, T. C. et. al. Clinicopathological analysis of c-kit expression in leiomyosarcomas of the uterine corpus[J]. 2003. Gynecol. Oncol. 91: 3-8. [2]. Driancourt, M. A., Reynaud, K., Cortvrindt, R. et. al. Roles of KIT and KIT LIGAND in ovarian function[J]. 2000. Rev. Reprod. 5: 143-152. [3]. Hutt, K. J., McLaughlin, E. A. and Holland, M. K. Kit ligand and c-Kit have diverse roles during mammalian oogenesis and folliculogenesis[J]. 2006.Mol. Hum. Reprod. 12: 61-69. [4]. Li, Y., Oral, O., Abe, K., Eto, K. et. al. The roles of pericystic cells and Sertoli cells in spermatogonial proliferation stimulated by some growth factors in organ culture of newt (Cynops pyrrhogaster) testis[J]. 2008. Gene. Comp. Endocrinol. 159: 80-87. [5]. Zhang, Z., Zhang, B., Nie, X., et. al. Transcriptome analysis and identification of genes related to immune function in skin of the Chinese brown frog[J]. 2009. Zool. Sci. 26: 80-86. [6]. The Pharmacopoeia Commission of PRC. Pharmacopoeia of the People’s Republic of China, English Edition 3rd ed[M]. Beijing. The People’s Medical Publishing House. 2000. [7]. Raucci, F. and Di Fiore, M. M. The c-kit receptor protein in the testis of green frog Rana esculenta: seasonal changes in relationship to testosterone titres and spermatogonial proliferation[J]. 2007. Reproduction 133: 51-60. 中国穿山甲与爪哇穿山甲甲片异速生长分析及其在 司法鉴定中的应用 * 周昭敏 , 赵