抗瘤中药调变结直肠癌细胞免疫抑制分子分泌比较研究.docVIP

抗瘤中药调变结直肠癌细胞免疫抑制分子分泌的比较研究 　作　者：李保红　崔澂　高峰　王润田　郑文广　张征峥 作者单位：050082　石家庄市，中国人民解放军白求恩军医学院训练部(李保红、崔澂、郑文广);河北省公安厅技术处(高峰);河北医科大学基础医学院免疫室(王润田、张征峥) 　　【摘要】 目的　比较研究不同类型抗瘤中药制剂对结直肠癌细胞分泌免疫抑制分子的影响。方法　分别制备三氧化二砷(As2O3)、川芎嗪(LHC)、黄芪(AMB)、氧化苦参碱(MOX)、猪苓多糖(PUPS)、青蒿琥酯(ART)等6种中药制剂作用后的小鼠结直肠癌细胞Colon 26 再培养上清;以不经中药制剂作用的相应上清为对照。定量酶联免疫吸附法(ELISA)测定上清中TGF-Β1、VEGF、IL-4、IL-6、IL-10和PGE2含量。结果　在6种中药制剂对Colon 26分泌免疫抑制分子的影响中，AMB对TGF-Β1的下调作用最强(55.75%);As2O3、LHC对IL-6的下调幅度均约5%，但作用时相不同;As2O3 对IL-10的下调作用最强(7.35%);对VEGF、IL-4及PGE2的影响之间差异无统计学意义(均P0.05)。结论　TGF-Β1可能是结直肠癌细胞分泌的优势免疫抑制分子，是各种中药制剂下调免疫抑制的重要靶分子;下调肿瘤细胞免疫抑制分子的分泌，应是中药制剂发挥抗瘤效应的机制之一。 　　【关键词】 中药制剂;结直肠肿瘤;Colon 26;小鼠;抑制因子，免疫 　　【Abstract】　Objective　To investigate the effects of different anti-tumor traditional Chinese medicines on the secretion of immunosuppressors from colorectal tumor cells (Colon 26) in vitro.Methods　The re-culture supernatants of Colon 26 (Murine colorectal tumor cells) were pre-treated by six different traditional Chinese medicines that included As2O3，Ligustrazine Hydrochloride (LHC)，Astragalus Mongholicus Bge (AMB)，Matrine N-Oxide (MOX)，Polyporus Umbellatus Polysacchride (PUPS) and Artesunate (ART)，and the corresponding supernatants of Colon26 without treatment by any medicine were used as controls.The concentrations of the six immunosuppressors including TGF-Β1，VEGF，IL-4，IL-6，IL-10 and PGE2 in supernatants were detected by quantitative ELISA.Results　Among the effects of six medicines on immunosuppressors，the down-regulating effect of AMB on TGF-Β1 was the strongest (55.75%)，and As2O3 and LHC down-regulated the levels of IL-6 by 5%，however，their effects and action time were different，the down-regulating effect of As2O3 on IL-10 was the strongest(7.35%)，but there were no significant differences in the effects of As2O3 on VEGF，IL-4 and PGE2 respectively (P0.05).Conclusion　TGF-Β1 is probably the dominant immunosuppressors secreted by colorectal tumor cells，which is the key target molecule in the down-regulating effects of traditiona