大白菜与诸葛菜属间杂交及GISH鉴定.pdfVIP

园　艺 　学 　报 　2009 , 36 ( 10) : 1473 - 1478 A cta Horticu lturae Sin ica 大白菜与诸葛菜属间杂交及 G ISH鉴定 乔海云 , 李 　菲 , 张淑江 , 章时蕃 , 孙 日飞 (中国农业科学院蔬菜花卉研究所 , 北京 100081) 摘 　要 : 以不同类型的大白菜与诸葛菜进行属间正反杂交 , 运用蕾期重复授粉及胚挽救技术 , 在大白 菜为母本的组合中获得了 31株幼苗 , GISH 鉴定表明有 16株具有预期的染色体数 , 2n = 22 , 为真杂种 ; 在 诸葛菜为母本的组合中没有得到杂种植株 。田间调查显示 , 杂种一代植株综合性状介于双亲之间 , 同时花 粉败育 。 关键词 : 大白菜 ; 诸葛菜 ; 属间杂交 ; GISH 中图分类号 : S 6341　　文献标识码 : A 　　文章编号 : 0513353X (2009) In tergener ic Hybr id iza tion of B ra ssica cam pestr is L. ssp. pek in en sis ( L our. ) O lsson and O rych oph ra gm u s vio la ceu s and G ISH Iden tif ica tion Q IAO H aiyun , L i Fei, ZHAN G Shuj iang, ZHAN G Sh ifan , and SUN R ifei ( Ins titu te of Veg etable and F low ers, Ch inese A cadem y of A g ricu ltu ra l S ciences, B eij ing 100081, Ch ina) A b stract: The intergeneric hybridization s between differen t B rass ica camp es tris L. ssp. p ek inens is (Lour. ) O lsson and O ry chop h ragm us violaceus were carried ou t. By succe ssive bud po llination and ovu le cu ltu re, B. camp es tris L. ssp. p ek inens is and O. violaceus were cro ssed recip rocally. W hen B. cam p es tris L. ssp. p ek inens is wa s u sed a s mother p lan ts, th irtyone p lan ts were ob tained, wh ile O. violaceus wa s u sed a s mother p lan ts, no p lan ts were re scued. Sixteen of the th irtyone p lan ts showed characteristic s of both p aren ts. G ISH iden tification revealed that sixteen of the th irtyone hyb rid p lan tswere real hyb rid s becau se they had the an ticip ative ch romo som e s (2n = 22 ) . The inve stigation in the field showed that m any characteristic s i