Controlofhydrogenphotoproductionbytheprotongradientgeneratedbycyclicelectronflow阅读笔记.docVIP

Control of hydrogen photoproduction by the proton gradient generated by cyclic electron flow in Chlamydomonas reinhardtii 题目翻译： 莱茵衣藻中通过环式电子传递产生的质子梯度控制光致产氢 作者： 摘要： 阅读笔记： 真核微藻的光致产氢，通过光合电子传递链和质体氢化酶之间的联系：“Hydrogen photoproduction by eukaryotic microalgae results from a connection between the photosynthetic electron transport chain and a plastidial hydrogenase. ” 藻类产氢，在自然条件下，通常是一种短暂的现象，通常被认为是一种避免光合电子传递链过度还原的一种保护安全阀：“Algal H2 production is a transitory phenomenon under most natural conditions, often viewed as a safety valve protecting the photosynthetic electron transport chain from overreduction. ” 分析莱茵衣藻的突变株，基于暗-光叶绿素荧光分析，分离出一株环绕PSI的环式电子传递CEF受损的突变株，由于其缺乏“质子梯度调节蛋白Proton Gradient Regulation Like1 (PGRL1) protein”：“From the colony screening of an insertion mutant library of the unicellular green alga Chlamydomonas reinhardtii based on the analysis of dark-light chlorophyll fluorescence transients, we isolated a mutant impaired in cyclic electron flow around photosystem I (CEF) due to a defect in the Proton Gradient Regulation Like1 (PGRL1) protein. ” 在好氧生活条件下，在突变株pgrl1中，非光化学淬灭（NPQ）显著降低：“Under aerobiosis, nonphotochemical quenching of fluorescence (NPQ) is strongly decreased in pgrl1.” 在厌氧生活条件下，在突变株pgrl1中的光致产氢显著增加，无论是在短期还是长期测量中（缺硫条件下）：“Under anaerobiosis, H2 photoproduction is strongly enhanced in the pgrl1 mutant, both during short-term and long-term measurements (in conditions of sulfur deprivation).” 根据NPQ和产生氢气的光依赖性，同时，在加入解耦联剂FCCP（uncoupling agent carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP)）时野生型的产氢量也增加，得出结论：CEF产生的质子梯度引起野生型中氢化酶的电子供应抑制，而在突变株pgrl1中是没有这种抑制的：“Based on the light dependence of NPQ and hydrogen production, as well as on the enhanced hydrogen production observed in the wild-type strain in the presence of the uncoupling agent carbonyl cyanide p-trifluoromethoxyphenylhydrazone, we conclude that the proton gradient generated by CEF provokes a strong inhibition of electron supply to the hydrogenase in the wild-type strain, which is released in the pgrl1