Identification of novel Trichoderma hamatum genes expressed during mycoparasitism using subtractive【荐】.pdf

FEMS Microbiology Letters 251 (2005) 105–112 Identification of novel Trichoderma hamatum genes expressed during mycoparasitism using subtractive hybridisation Margaret A. Carpenter a,*, Alison Stewart b, Hayley J. Ridgway a a Bio-Protection and Ecology Division, P.O. Box 84, Lincoln University, Canterbury, New Zealand b National Centre for Advanced Bio-Protection Technologies, P.O. Box 84, Lincoln University, Canterbury, New Zealand Received 4 May 2005; received in revised form 10 July 2005; accepted 26 July 2005 First published online 9 August 2005 Edited by M.R. Soria Abstract Subtractive hybridisation was used to target novel genes involved in the mycoparasitic interaction of the biocontrol agent Trich- oderma hamatum with the phytopathogen Sclerotinia sclerotiorum. Nineteen novel T. hamatum genes were identified that showed increased expression during mycoparasitism compared to a T. hamatum control. Sequence analysis revealed some cDNA fragments had similarity to known fungal or bacterial genes whereas others had no similarity to any genes previously described. Only one of the novel genes has been characterised in another Trichoderma species, the Trichoderma reesei hex1gene. The proteins encoded by the novel genes included three monooxygenases, a metalloendopeptidase, a gluconate dehydrogenase, an endonuclease and a proton ATPase. 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. Keywords: Trichoderma hamatum; Mycoparasitism; Subtractive hybridisation; Biological control; SSH 1. Introduction