放射性皮肤损伤动物模型制备.pdf

放射性皮肤损伤动物模型制备及病变规律1 1 2 2 1 1 1 1 陈强 谢兵 姜永莉 孟洪琪 张东威 宁康 范洪学 * [摘要] 目的 制造出符合临床病变规律的放射性皮肤损伤动物模型 利用此模型对放 射性皮肤损伤形成的机理进行研究 方法 使用深部X 线治疗机对实验动物进行外照射 经过一段时间的病理变化形成急性 度放射性皮肤损伤模型 对动物模型受照皮肤的病变过 程进行观察 并取不同时间点的创面皮肤进行核酸测定和病理检查 结果 动物模型的皮 肤病变规律与临床相似 损伤皮肤中核酸含量与正常皮肤比较差别较大 P0.05 常规病理 标本与电镜标本都表明 微循环障碍是导致放射性皮肤损伤难以治愈的根本原因 结论 利 用X 射线对实验动物进行局部照射 制成的放射性皮肤损伤模型 其病变规律与临床病例接 近 可用于进行放射性皮肤损伤的研究 并为以后的工作奠定了基础 [关键词] 放射性 皮肤损伤 动物模型 病变规律 Preparation and pathological changes rule of skin radiation damage in animal model 1 2 2 1 CHEN Qiang XIE Bing JIANG Yong-li MENG Hong-qi 1 1 1 ZHANGDong-wei NING Kang FAN Hong-xue * 1.School of Public Health, Ji Lin University, ChangChun,130021 2. Jilin Entry-exit Inspection and Quarantine Bureau of P.R.C. Changchun international travel healthcare center ChangChun,130062 [Abstract] Objective To prepare the animal model of skin radiation damage that mach the clinical pathological changes rule, study the mechanism of skin radiation damage using this model.Method Guinea pigs were irradiated using deep X-ray therapy machine to make acute III degree skin radiation damage model after a time of pathological changes. The irradiated skin was observed, followed by RNA check up and pathological examine at different time spot. Result The skin pathological changes of model are similar to clinical pathological changes of patient. Damaged skin possesses fewer RNA than normal skin (P0.05). According to pathological and electron microscope check up, micro circulation block is the main reason that result in the difficulty to cure the skin radiation damage. Conclusion After irrad