东北农业大学学报模板.docVIP

中文写作模板 整篇文档正文5号宋体，一级标题4号宋体，1.5倍行距。 XXXXXXX的研究 xxx，xxx2*，x　x3 （ 1. XXX大学学院，哈尔滨　150010；2.农业大学XXX学院，哈尔滨　150030； 3. XXXXXXXX学院，黑龙江　大庆　161090 ） 　　摘　要：利用长期施用长残留除草剂咪唑乙烟酸的土壤，采用高压富集的方法，从两种培养液中共分离出3株细菌，根据菌株的形态特性和生理生化特征，对此3个菌株进行了初步鉴定：菌株X为丙酸杆菌属（Propionibacterium）；菌株Y为海球菌属（Marinococcus）；菌株Z为酸单胞杆菌属（Acidomonas）；菌株X、Y和Z的最适生长温度分别是30、30和35 ℃；菌株X和Z在中性偏酸的培养基中生长较好，菌株Y在偏碱培养基中生长较好；菌株X、Y、Z生长最适合的葡萄糖含量分别为0.5%、1%和0.1%；菌株Y生长最适咪唑乙烟酸的浓度是10 mg a.i.·L-1，菌株X和Z生长最适咪唑乙烟酸的浓度是20 mg a.i.·L-1。 　　 关键词：咪唑乙烟酸；降解菌；富集分离；鉴定；生长特性 　　 中图分类号：S767.5；X172　　　　文献标志码：A Separation identification and growth characters of herbicide imazethapyr degrading-bacteria XXX Xxxx1， 2， 3 （1. College of , Xxx, Harbin 150010, China; 2. College of Xe, Xxxxx, Harbin 150030, China; 3. Xxxx of Xxxxxxx, Daoqin Heilongjiang 161090, China) 　　Abstract: Using the high pressure enrichment method, three bacteria strains in two culture media were separated from soil, which contained the herbicide imazethapyr and were long polluted by the pesticide. By batch culture with clomazone as a sole carbon, strain X was identified as Propionibacterium, strain Y was identified as Marinococcu, and strain Z was identified as Acidomonas according to the morphological and physiological char-acters. The optimal temperature for growth of X, Y and Z were 30, 30, and 35℃, respectively. The growth of strain X and Z was good in neutral and weak acidic culture media, but strain Y was appropriate to growth under alkalescence; the optimal sugar contents for growth of X, Y and Z were 0.5%, 1% and 0.1%, respectively. The optimal initial concentration for imazethapyr of Y was 10 mg a.i.·L-1, and that for X and Z was 20 mg a.i.·L-1. 　　Key words: imazethapyr; degrading microorganisms; enrichment separation; identification; growth characters咪唑乙烟酸（Imazethapyr）是由美国氰胺公司（现归并为德国BASF公司）于80年代初开发研制的大豆田选择性苗前、苗后早期除草剂，主要防除稗草、反枝苋等一年生禾本科杂草和阔叶杂草。咪唑乙烟酸具有杀草谱广、选择性强、活性高等优点，它可以被植物的叶片和根系吸收，在木质部与韧皮部内传导，积累于分生组织[1-2]。其作用方式是抑制乙酰乳酸合成酶（Acetolactate Synthase，ALS），破坏蛋白质合成，使植物生长受抑制而死亡。而豆科植物吸收咪唑乙烟酸后，在体内很