应用PCRSSCP银染技术检测HBV基因点突变.pdfVIP

·216· 盛国光1 李瀚昊1 冉瑞琼2 王伯祥1 张建军1 晏雪生1 明安萍1 1．湖北中医学院附属医院(武汉，430061)2．上海医科大学 摘 要 目的：为了对乙型肝炎患者HBV基因突变进行大样本大面积筛检，建立简便可靠的检测HBV基因突变 的方法。方法：采用单链构象多态性(sSCP)银染技术，检测HBVPre—c基因突变。结果：在10份HBeAg阳性标本 1898位发生了G—A变异，出现TAG终止密码突变。结论：PCR—SSCP银粢技术检测HBVPre—C基因点突变有很高 的特异性和敏感性，该方法稳定、简便、快速，能基本满足临床大面积大样本筛检的需要。 关键词PCRSSCP银染技术HBV基因突变 theMutationofHBV PCR—SSCPandSilVer Detectihg byUsing Staining Technique S爿_ENG H口雄mf挖，RAN J，lsfif摊抬。丸L而Pr皿sP口sPs。日曲ei Gl‘Dg狂口雄g，LJ R“幻fD雄g，甜“． oZ厶曙已∥粥M(W“妇挖， 430061) muta— Abtncter establishaconvenientandreliable methodfor the Objec“ve：To detecting presc“ptivescreening ona strandconformation of chain tionof ofHBV scale．Methods：The polymorphismanalysispolymerase gene larg single andsilver wereusedfor the mutationof B reaction hepatitis (PCR—SSCP)productsstainingtechnique detectingprecore Inthe ten serum of were forHBVDNA virus．R髓ul协l seropositive experiment，amongpositive samplesHBeAg，eight thetwoofthemexhibitedmutationofPre—C ofHBVfurtherdetection detectionPCR，andthen by using by uising gene themutationthese wereincharacterofnon codewasdemon- SSCP Thefactthat for two technique． samples stopping the of were forHBV strateddirect anti—HBe，twelve by