菊属野生种,栽培菊花及种间杂种的RAPD分析.pdfVIP

南京农业大学学报 　2004 , 27 (3) : 29～33 　 Journal of N anjin g A gricultural U niversity 菊属野生种、栽培菊花及种间杂种的 RAPD 分析 李辛雷, 陈发棣 (南京农业大学园艺学院 , 江苏 南京 210095) 摘要 : 以菊属野生种、栽培菊花及种间杂种为试材, 采用 RAPD 方法研究其亲缘关系。从 40 条随机引物中筛选出 15 条 , 对 23 份试材进行扩增 , 共产生 153 条带 , 平均每条引物扩增出 102 条带 , 多态性条带占 771 % 。通过遗传相似性 矩阵及 U PGMA 聚类分析 , 结合杂交结实性与幼胚拯救成苗率 , 认为 : 父母本亲缘关系近的遗传距离较小 , 杂交亲和性 好 , 结实率较高 , 幼胚拯救易成功 ; 反之杂交亲和性差 , 难以结实 , 幼胚拯救成苗率低。根据以上数据结合前人研究结 果 , 对栽培菊花的起源演化进行了探讨。 关键词 : 菊属 ; RAPD ; 亲缘关系 ; 杂交亲和性 ; 幼胚拯救 中图分类号: S6821+ 1 　　　文献标识码 : A 　　　文章编号 : 1000 2030 (2004) 03 0029 05 RAPD analysis of wild specie s , cultivars and interspecific hybrids in Dendranthema L I Xinlei , CHEN Fadi (College of Horticulture , Nanjin g Agricultural University , Nanjin g 210095 , China) Abstract : The relationship of 23 wild species , cultivars and interspecific hybrids in Dendranthem a was studied by RAPD analy sis. 15 random primers were screened to detect polymorphism among the total materials used. Of the 153 bands produced , 771 % were polymorphic. Each primer amplified an average of 102 bands. The results from pairwise similarity and U PGMA cluster analysis , combining with the fecundity of hybridization and rate of plantlet of embryo rescue , suggested that when parents have closer relationship , the genetic distance was closer , the compatibility of hybridization was better , fecundity was higher and embryo rescue was easier. When parents have further relationship , the results were contrary. According to the RAPD analysis and previous research , the