天津野生鲫鱼卵黄蛋白原的研究.pdfVIP

中国环境科学 2003,23(3) ：281~284 China Environmental Science 天津野生鲫鱼卵黄蛋白原的研究 * 胡建英,安立会,孙晓航,张海峰,邓宝山 (北京大学环境学院,北京 100871) 摘要：用HPLC 方法提纯经雌二醇诱导的鲫鱼卵黄蛋白原,用SDS凝胶电泳测定其分子量为160Kda 左右.在建立卵黄蛋白原(VTG) 的HPLC 分析方法(检测下限为0.025mg/mL)的基础上,对北京排污河天津段的野生和养殖鲫鱼血浆中的VTG 进行检测.在所捕获的12 尾 野生鲫鱼中(其中2 尾为雄性鲫鱼),都检测出了VTG,浓度范围为 0.284~5.971mg/mL;对同期捕获的35 尾天津养殖鲫鱼包括1 尾雄性鲫鱼 中的VTG 也进行了检测,浓度范围为0.119~0.250mg/mL.而同期在实验室饲养的雌雄鲫鱼血浆中,均未检测出VTG.结果表明,北京排污河 (天津段)的野生鲫鱼受到类雌激素物质的污染. 关键词：内分泌干扰物；鲫鱼；卵黄蛋白原；生物标志物 中图分类号：X703.5 文献标识码：A 文章编号：1000-6923(2003)03-0281-04 Investigation of vitellogenin in wild crucian (Carassius auratus) Tianjin. HU Jian-ying, AN Li-hui, SUN Xiao-hang, ZHANG Hai-feng, DENG Bao-shan (College of Environmental Science, Peking University, Beijing 100871, China). China Environmental Science, 2003,23(3)：281~284 Abstract：Vitellogenin (VTG) in crucian (Carassius auratus) induced by injection of 17â-estradiol was purified using HPLC method, and its molecular weight was determined to be about 160Kda using SDS. Based on the HPLC analysis method of vitellogenin established (its low limit of detection was 0.025ìg/mL), the VTG in the plasma of wild and cultured crucians from Beijingpaiwu River in Tianjin area was detected. Vitellogenin was detected in all of 12 caught wild fishes , (including 2 male fishes) and the concentration ranged from 0.284 to 5.971mg/mL. Vitellogenin was also detected in all of 35 cultured fishes including 1 male, catched in same period and the concentration ranged from 0.119 to 0.250mg/mL; but no vitellogenin was detected in both male and female cultured in laboratory in same period. The wild crucian from Beijingpaiwu River in Tianjin was contaminated by estrogenic matter. Key words ：endocrine disruptors ；crucian；vitellogenin ；biomarker 在低浓度内分泌干扰物质的长期暴露下导 VTG 已经成为检测水生生物类雌激素暴露下的 致水生生物生殖异常现象已经引起科学界的重 一个重